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Literature summary for 2.7.7.38 extracted from

  • Ghalambor, M.A.; Heath, E.H.
    The biosynthesis of cell wall lipopolysaccharide in Escherichia coli. IV. Purification and properties of cytidine monophosphate 3-deoxy-D-manno-octulosonate synthetase (1966), J. Biol. Chem., 241, 3216-3221.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
GSH
-
Escherichia coli

General Stability

General Stability Organism
repeated freezing and thawing results in substantial loss in activity Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.22
-
CTP pH 8.0, 37°C Escherichia coli
0.8
-
3-deoxy-D-manno-octulosonate pH 8.0, 37°C Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ absolute requirement for a divalent cation Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CTP + 3-deoxy-D-manno-octulosonate Escherichia coli biosynthesis of cell wall lipopolysaccharide diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
r
CTP + 3-deoxy-D-manno-octulosonate Escherichia coli 0111-B4 biosynthesis of cell wall lipopolysaccharide diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
r

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli 0111-B4
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
50
-
-
Escherichia coli

Storage Stability

Storage Stability Organism
-1°C, enzyme preparation is most satisfactorily stored since inactivation of the enzyme under this condition is negligible over a period of several months Escherichia coli
-20°C,partially purified enzyme relatively stable, approximately 20% of the activity is lost after storage for 1 year Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CTP + 3-deoxy-D-manno-octulosonate
-
Escherichia coli diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
r
CTP + 3-deoxy-D-manno-octulosonate biosynthesis of cell wall lipopolysaccharide Escherichia coli diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
r
CTP + 3-deoxy-D-manno-octulosonate
-
Escherichia coli 0111-B4 diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
r
CTP + 3-deoxy-D-manno-octulosonate biosynthesis of cell wall lipopolysaccharide Escherichia coli 0111-B4 diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9 about half-maximal activity at pH 7.0 and pH 8.4 Escherichia coli