Activating Compound | Comment | Organism | Structure |
---|---|---|---|
GSH | - |
Escherichia coli |
General Stability | Organism |
---|---|
repeated freezing and thawing results in substantial loss in activity | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
CTP | pH 8.0, 37°C | Escherichia coli | |
0.8 | - |
3-deoxy-D-manno-octulosonate | pH 8.0, 37°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | absolute requirement for a divalent cation | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + 3-deoxy-D-manno-octulosonate | Escherichia coli | biosynthesis of cell wall lipopolysaccharide | diphosphate + CMP-3-deoxy-D-manno-octulosonate | - |
r | |
CTP + 3-deoxy-D-manno-octulosonate | Escherichia coli 0111-B4 | biosynthesis of cell wall lipopolysaccharide | diphosphate + CMP-3-deoxy-D-manno-octulosonate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli 0111-B4 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-1°C, enzyme preparation is most satisfactorily stored since inactivation of the enzyme under this condition is negligible over a period of several months | Escherichia coli |
-20°C,partially purified enzyme relatively stable, approximately 20% of the activity is lost after storage for 1 year | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + 3-deoxy-D-manno-octulosonate | - |
Escherichia coli | diphosphate + CMP-3-deoxy-D-manno-octulosonate | - |
r | |
CTP + 3-deoxy-D-manno-octulosonate | biosynthesis of cell wall lipopolysaccharide | Escherichia coli | diphosphate + CMP-3-deoxy-D-manno-octulosonate | - |
r | |
CTP + 3-deoxy-D-manno-octulosonate | - |
Escherichia coli 0111-B4 | diphosphate + CMP-3-deoxy-D-manno-octulosonate | - |
r | |
CTP + 3-deoxy-D-manno-octulosonate | biosynthesis of cell wall lipopolysaccharide | Escherichia coli 0111-B4 | diphosphate + CMP-3-deoxy-D-manno-octulosonate | - |
r |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9 | about half-maximal activity at pH 7.0 and pH 8.4 | Escherichia coli |