Application | Comment | Organism |
---|---|---|
medicine | ebosin is an exopolysaccharide with antagonist activity for interleukin-1 receptor in vitro and remarkable anti-rheumatic arthritis activity in vivo | Streptomyces sp. 139 |
Cloned (Comment) | Organism |
---|---|
the ste17 gene coding region is cloned into the plasmid pET30a for expression in Escherichia coli BL21 cells | Streptomyces sp. 139 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.142 | - |
CTP | - |
Streptomyces sp. 139 | |
0.429 | - |
alpha-D-glucose 1-phosphate | - |
Streptomyces sp. 139 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30900 | - |
including 2.5 kDa of His-tag originated from pET30a, determined by SDS-PAGE | Streptomyces sp. 139 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + alpha-D-glucose 1-phosphate | Streptomyces sp. 139 | - |
diphosphate + CDP-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. 139 | Q8KN04 | - |
- |
Purification (Comment) | Organism |
---|---|
on a Ni-NTA His-Bind resin column | Streptomyces sp. 139 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + alpha-D-glucose 1-phosphate | - |
Streptomyces sp. 139 | diphosphate + CDP-glucose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha-D-glucose-1-phosphate cytidylyltransferase | - |
Streptomyces sp. 139 |
CDP-D-glucose synthase | - |
Streptomyces sp. 139 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
activity assay | Streptomyces sp. 139 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
activity assay | Streptomyces sp. 139 |