Cloned (Comment) | Organism |
---|---|
- |
Pyrococcus abyssi |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | nucleotidyl-transferase activity of Rep75 is Mn2+ dependent, no nucleotidyl-transferase activity with Mg2+ | Pyrococcus abyssi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus abyssi | O54003 | - |
- |
Pyrococcus abyssi GE5 / CNCM I-1302 / DSM 25543 | O54003 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus abyssi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dATP + DNAn | terminal transferase activity at the 3'-OH extremity of the nicking site | Pyrococcus abyssi | diphosphate + DNAn+1 | - |
? | |
dATP + DNAn | terminal transferase activity at the 3'-OH extremity of the nicking site | Pyrococcus abyssi GE5 / CNCM I-1302 / DSM 25543 | diphosphate + DNAn+1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Rep75 | - |
Pyrococcus abyssi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
the nucleotidyl-transferase activity of Rep75 is less thermophilic than its nicking activity (105°C) | Pyrococcus abyssi |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
1 h, no loss of activity after 1 h | Pyrococcus abyssi |