General Stability | Organism |
---|---|
not stable in urea, SDS and organic solvents | Bos taurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | Co2+ is the best activator in elongation of chains with dCTP or dTTP | Mus musculus | |
Co2+ | Co2+ is the best activator in elongation of chains with dCTP or dTTP | Homo sapiens | |
Co2+ | Co2+ is the best activator in elongation of chains with dCTP or dTTP | Bos taurus | |
Mg2+ | activates | Mus musculus | |
Mg2+ | activates | Homo sapiens | |
Mg2+ | activates | Bos taurus | |
Mn2+ | - |
Mus musculus | |
Mn2+ | - |
Bos taurus | |
Mn2+ | human enzyme from leukemic cells shows maximal activity with Mn2+ as divalent cation | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
- |
Mus musculus |
60000 | - |
- |
Homo sapiens |
79000 | - |
- |
Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNA | Mus musculus | hypothesis: terminal transferase acts as a somatic mutator, diversifying the amino acid sequence in the variable region of immunoglobulin molecules by changing one of the nucleotides | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNA | Homo sapiens | hypothesis: terminal transferase acts as a somatic mutator, diversifying the amino acid sequence in the variable region of immunoglobulin molecules by changing one of the nucleotides | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNA | Bos taurus | hypothesis: terminal transferase acts as a somatic mutator, diversifying the amino acid sequence in the variable region of immunoglobulin molecules by changing one of the nucleotides | diphosphate + DNAn+1 | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
additional information | not stable in organic solvents | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Homo sapiens | - |
- |
- |
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a 2'-deoxyribonucleoside 5'-triphosphate + DNAn = diphosphate + DNAn+1 | mechanism | Mus musculus | |
a 2'-deoxyribonucleoside 5'-triphosphate + DNAn = diphosphate + DNAn+1 | mechanism | Homo sapiens | |
a 2'-deoxyribonucleoside 5'-triphosphate + DNAn = diphosphate + DNAn+1 | mechanism | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bone marrow | - |
Mus musculus | - |
bone marrow | - |
Homo sapiens | - |
bone marrow | - |
Bos taurus | - |
thymus | - |
Mus musculus | - |
thymus | - |
Homo sapiens | - |
thymus | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
overview assay methods | Mus musculus |
additional information | - |
overview assay methods | Homo sapiens |
additional information | - |
overview assay methods | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dATP + DNAn | - |
Bos taurus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNA | hypothesis: terminal transferase acts as a somatic mutator, diversifying the amino acid sequence in the variable region of immunoglobulin molecules by changing one of the nucleotides | Mus musculus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNA | hypothesis: terminal transferase acts as a somatic mutator, diversifying the amino acid sequence in the variable region of immunoglobulin molecules by changing one of the nucleotides | Homo sapiens | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNA | hypothesis: terminal transferase acts as a somatic mutator, diversifying the amino acid sequence in the variable region of immunoglobulin molecules by changing one of the nucleotides | Bos taurus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | primer required | Mus musculus | diphosphate + DNAn+1 | - |
ir | |
deoxynucleoside triphosphate + DNAn | primer required | Homo sapiens | diphosphate + DNAn+1 | - |
ir | |
deoxynucleoside triphosphate + DNAn | primer required | Bos taurus | diphosphate + DNAn+1 | - |
ir | |
deoxynucleoside triphosphate + DNAn | longer chains interact well with the enzyme, but synthesis rate decreases | Mus musculus | diphosphate + DNAn+1 | - |
ir | |
deoxynucleoside triphosphate + DNAn | longer chains interact well with the enzyme, but synthesis rate decreases | Homo sapiens | diphosphate + DNAn+1 | - |
ir | |
deoxynucleoside triphosphate + DNAn | longer chains interact well with the enzyme, but synthesis rate decreases | Bos taurus | diphosphate + DNAn+1 | - |
ir | |
deoxynucleoside triphosphate + DNAn | primer: oligonucleotides containing at least 3 phosphate groups and a free 3'-hydroxyl end | Mus musculus | diphosphate + DNAn+1 | - |
ir | |
deoxynucleoside triphosphate + DNAn | primer: oligonucleotides containing at least 3 phosphate groups and a free 3'-hydroxyl end | Homo sapiens | diphosphate + DNAn+1 | - |
ir | |
deoxynucleoside triphosphate + DNAn | primer: oligonucleotides containing at least 3 phosphate groups and a free 3'-hydroxyl end | Bos taurus | diphosphate + DNAn+1 | - |
ir | |
deoxynucleoside triphosphate + DNAn | reaction is not template directed | Mus musculus | diphosphate + DNAn+1 | - |
ir | |
deoxynucleoside triphosphate + DNAn | reaction is not template directed | Homo sapiens | diphosphate + DNAn+1 | - |
ir | |
deoxynucleoside triphosphate + DNAn | reaction is not template directed | Bos taurus | diphosphate + DNAn+1 | - |
ir | |
deoxynucleoside triphosphate + DNAn | as the initiator chain length is increased to 5-7 nucleotides the polymerization rate also increases | Mus musculus | diphosphate + DNAn+1 | - |
ir | |
deoxynucleoside triphosphate + DNAn | as the initiator chain length is increased to 5-7 nucleotides the polymerization rate also increases | Homo sapiens | diphosphate + DNAn+1 | - |
ir | |
deoxynucleoside triphosphate + DNAn | as the initiator chain length is increased to 5-7 nucleotides the polymerization rate also increases | Bos taurus | diphosphate + DNAn+1 | - |
ir |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Homo sapiens |
monomer | 1 * 60000 | Mus musculus |
monomer | 1 * 79000 | Bos taurus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
not stable above | Bos taurus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.833 | - |
dATP | - |
Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
- |
Bos taurus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
stable at | Bos taurus |