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Literature summary for 2.7.7.31 extracted from
- Deoxynucleotide-polymerizing enzymes of calf thymus gland. V. Homogeneous terminal deoxynucleotidyl transferase (1971), J. Biol. Chem., 246, 909-916.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 8000 | - |
1 * 8000 (alpha) + 1 * 26500 (beta), SDS-PAGE | Bos taurus |
| 26500 | - |
1 * 8000 (alpha) + 1 * 26500 (beta), SDS-PAGE | Bos taurus |
| 32360 | - |
equilibrium sedimentation | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| calf | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| thymus | - |
Bos taurus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 40% loss of activity after 7 months | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| deoxynucleoside triphosphate + DNAn | primer required | Bos taurus | diphosphate + DNAn+1 | - |
? |  |
| deoxynucleoside triphosphate + DNAn | reaction is not template directed | Bos taurus | diphosphate + DNAn+1 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 8000 (alpha) + 1 * 26500 (beta), SDS-PAGE | Bos taurus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
assay at | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
assay at | Bos taurus |
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Release 2023.1 (January 2023)
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