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Literature summary for 2.7.7.30 extracted from

  • Quirk, S.
    Crystallization and preliminary X-ray characterization of the Vitis vinifera fucokinase:GDP-fucose pyrophosphorylase (2012), Acta Crystallogr. Sect. F, 68, 1109-1112.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expresssion in Escherichia coli Vitis vinifera

Crystallization (Commentary)

Crystallization (Comment) Organism
to 2.6 A resolution. Space group P41212, a single enzyme monomer per asymmetric unit Vitis vinifera

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
GTP pH not specified in the publication, temperature not specified in the publication Vitis vinifera
0.8
-
ATP pH not specified in the publication, temperature not specified in the publication Vitis vinifera

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
118800
-
x * 118800, calculated Vitis vinifera

Organism

Organism UniProt Comment Textmining
Vitis vinifera D7UCW9
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein with maltose-binding protein affinity tag Vitis vinifera

Storage Stability

Storage Stability Organism
-20°C, recombinant protein with maltose-binding protein affinity tag, stable for at least three months Vitis vinifera

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + beta-L-fucose 1-phosphate
-
Vitis vinifera ADP-L-fucose + diphosphate
-
?
GTP + beta-L-fucose 1-phosphate
-
Vitis vinifera GDP-L-fucose + diphosphate
-
?

Subunits

Subunits Comment Organism
? x * 118800, calculated Vitis vinifera

Synonyms

Synonyms Comment Organism
FKP
-
Vitis vinifera

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.22
-
ATP pH not specified in the publication, temperature not specified in the publication Vitis vinifera
1.55
-
GTP pH not specified in the publication, temperature not specified in the publication Vitis vinifera