Literature summary for 2.7.7.3 extracted from

Timofeev, V.; Chupova, L.; Esipov, R.; Kuranova, I.
Crystallization and preliminary X-ray diffraction study of phosphopantetheine adenylyltransferase from M. tuberculosis crystallizing in space group P3_2 (2015), Crystallogr. Rep., 60, 682-684.

No PubMed abstract available

Crystallization (Commentary)

Crystallization (Comment)	Organism
enzyme crystals are grown in microgravity by the capillary counter-diffusion method through a gel layer, mixing of 10 mg/ml protein in 10 mM HEPES, pH 8.0, 0.15 M NaCl, and 14 mM ATP, with reservoir solution containing 0.1 M NaAc, pH 5.0, 10 mM MgCl2, 5 mM HEPES, pH 8.0, 0.075 M NaCl, 14 mM ATP, and 1.1 M ammonium sulfate, X-ray diffraction structure determination and analysis at 2.0 A resolution, molecular replacement method	Mycobacterium tuberculosis

Crystallization (Comment)

Organism

enzyme crystals are grown in microgravity by the capillary counter-diffusion method through a gel layer, mixing of 10 mg/ml protein in 10 mM HEPES, pH 8.0, 0.15 M NaCl, and 14 mM ATP, with reservoir solution containing 0.1 M NaAc, pH 5.0, 10 mM MgCl2, 5 mM HEPES, pH 8.0, 0.075 M NaCl, 14 mM ATP, and 1.1 M ammonium sulfate, X-ray diffraction structure determination and analysis at 2.0 A resolution, molecular replacement method

Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
17028	-	-	Mycobacterium tuberculosis

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	P9WPA5	-	-

Subunits

Subunits	Comment	Organism
hexamer	6 * 17028, sequence calculation and crystal structure analysis, six enzyme subunits form a hexamer per asymmetric unit, the hexamer is a biologically active form of phosphopantetheine adenylyltransferase from Mycobacetrium tuberculosis	Mycobacterium tuberculosis

Synonyms

Synonyms	Comment	Organism
phosphopantetheine adenylyltransferase	-	Mycobacterium tuberculosis
PPAT	-	Mycobacterium tuberculosis

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Release 2023.1 (January 2023)