Literature summary for 2.7.7.3 extracted from

Miller, J.R.; Ohren, J.; Sarver, R.W.; Mueller, W.T.; de Dreu, P.; Case, H.; Thanabal, V.
Phosphopantetheine adenylyltransferase from Escherichia coli: Investigation of the kinetic mechanism and role in regulation of CoA biosynthesis (2007), J. Bacteriol., 189, 8196-8205.

Search for more literature for 2.7.7.3

Application

Application	Comment	Organism
medicine	PPAT is a target for antibacterial drug discovery	Escherichia coli

Cloned(Commentary)

Cloned (Comment)	Organism
expression of His6-tagged and wild-type PPAT in Escherichia coli BL21(DE3)	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
at pH 5.0, 100 mM sodium acetate, pH 5.0, 1.1 M ammonium sulfate, 200 mM NaCl	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
R106A	pantothenate kinase (PanK) mutant	Escherichia coli

Inhibitors

Inhibitors	Comment	Organism	Structure
CoA	-	Escherichia coli
dephospho-CoA	-	Escherichia coli
diphosphate	-	Escherichia coli
additional information	no inhibition is observed with AMPCPP, ATP-gamma-S, ADPPNP, or ADP at concentrations up to 1 mM and although no inhibition is observed with acetyl-CoA, desulfo-CoA, and palmitoyl-CoA at concentrations up to 500 mM	Escherichia coli
phosphopantetheine	-	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0047	-	phosphopantetheine	-	Escherichia coli
0.0047	-	phosphopantetheine	forward reaction	Escherichia coli
0.017	-	dephospho-CoA	-	Escherichia coli
0.017	-	dephospho-CoA	reverse reaction	Escherichia coli
0.22	-	ATP	-	Escherichia coli
0.22	-	Mg-ATP	forward reaction	Escherichia coli
0.23	-	diphosphate	-	Escherichia coli
0.23	-	diphosphate	reverse reaction	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
phosphopantetheine + Mg-ATP	Escherichia coli	essential hexameric enzyme that catalyzes the penultimate step in coenzyme A biosynthesis	dephospho-CoA + diphosphate	-	r
phosphopantetheine + MgATP2- + 4 H+	Escherichia coli	coenzyme A (CoA) biosynthesis	dephospho-CoA + diphosphate + Mg2+	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
copurification as hexamer with 0.5 equivalents of CoA tightly bound per PPAT monomer	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + pantetheine 4'-phosphate	-	Escherichia coli	diphosphate + 3'-dephospho-CoA	-	?
phosphopantetheine + Mg-ATP	-	Escherichia coli	dephospho-CoA + diphosphate	-	r
phosphopantetheine + Mg-ATP	essential hexameric enzyme that catalyzes the penultimate step in coenzyme A biosynthesis	Escherichia coli	dephospho-CoA + diphosphate	-	r
phosphopantetheine + MgATP2- + 4 H+	coenzyme A (CoA) biosynthesis	Escherichia coli	dephospho-CoA + diphosphate + Mg2+	-	?

Subunits

Subunits	Comment	Organism
homohexamer	6*17800, the dyad axis of the hexamer divides the protein into two asymmetrical trimers	Escherichia coli
homohexamer	taken from literature, 6*17800Da	Escherichia coli

Synonyms

Synonyms	Comment	Organism
phosphopantetheine adenylyltransferase	-	Escherichia coli
PPAT	-	Escherichia coli

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0084	-	phosphopantetheine	Substrate: dephospho-CoA, Cosubstrate: diphosphate (1.6 mM)	Escherichia coli
0.011	-	CoA	Substrate: dephospho-CoA, Cosubstrate: diphosphate (1.6 mM)	Escherichia coli
0.014	-	CoA	Substrate: phosphopantetheine, Cosubstrate: ATP (300 microM)	Escherichia coli
0.07	-	CoA	Substrate: ATP, Cosubstrate: phosphopantetheine (300 microM)	Escherichia coli
0.117	-	CoA	Substrate: Pyrophosphate, Cosubstrate: dephospho-CoA (300 microM)	Escherichia coli
0.12	-	diphosphate	Substrate: ATP, Cosubstrate: phosphopantetheine (300 microM)	Escherichia coli
0.121	-	dephospho-CoA	Substrate: phosphopantetheine, Cosubstrate: ATP (3.0 mM)	Escherichia coli
0.131	-	phosphopantetheine	Substrate: diphosphate, Cosubstrate: dephospho-CoA (500 microM)	Escherichia coli
0.192	-	dephospho-CoA	Substrate: ATP, Cosubstrate: phosphopantetheine (300 microM)	Escherichia coli

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Release 2023.1 (January 2023)