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Literature summary for 2.7.7.3 extracted from

  • Nalezkova, M.; de Groot, A.; Graf, M.; Gans, P.; Blanchard, L.
    Overexpression and purification of Pyrococcus abyssi phosphopantetheine adenylyltransferase from an optimized synthetic gene for NMR studies (2005), Protein Expr. Purif., 39, 296-306.
    View publication on PubMed
Search for more literature for 2.7.7.3

Cloned(Commentary)

Cloned (Comment) Organism
expression of native gene in Escherichia coli BL21(DE3) not possible, expression of optimized gene in Escherichia coli BL21(DE3) as His-tag fusion protein Pyrococcus abyssi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19990
-
 MALDI-TOF mass spectrometry of His-tag fusion protein Pyrococcus abyssi
19992
-
 1 * 20000, SDS-PAGE of His-tag fusion protein, circular dichroism, and 2D NMR experiments, 1 * 19992, MALDI-TOF mass spectrometry Pyrococcus abyssi
20000
-
 SDS-PAGE of His-tag fusion protein Pyrococcus abyssi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + pantetheine 4'-phosphate Pyrococcus abyssi rate-limiting penultimate step in coenzyme A biosynthesis diphosphate + 3'-dephospho-CoA
-
 r

Organism

Organism UniProt Comment Textmining
Pyrococcus abyssi Q9UYT0
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein using His-tag Pyrococcus abyssi

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.07
-
 reverse reaction using the purified His-tag fusion protein Pyrococcus abyssi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + pantetheine 4'-phosphate
-
 Pyrococcus abyssi diphosphate + 3'-dephospho-CoA
-
 r
ATP + pantetheine 4'-phosphate rate-limiting penultimate step in coenzyme A biosynthesis Pyrococcus abyssi diphosphate + 3'-dephospho-CoA
-
 r

Subunits

Subunits Comment Organism
monomer 1 * 20000, SDS-PAGE of His-tag fusion protein, circular dichroism, and 2D NMR experiments, 1 * 19992, MALDI-TOF mass spectrometry Pyrococcus abyssi