Protein Variants | Comment | Organism |
---|---|---|
P103A | mutation in loop Pro103-Arg115, mutant protein displays altered kinetic profiles, primarily a lack of response to fructose-1,6-bisphosphate | Escherichia coli |
Q106A | mutation in loop Pro103-Arg115, mutant protein displays altered kinetic profiles, primarily a lack of response to fructose-1,6-bisphosphate | Escherichia coli |
R107A | mutation in loop Pro103-Arg115, mutant protein displays altered kinetic profiles, primarily a lack of response to fructose-1,6-bisphosphate | Escherichia coli |
R115A | mutation in loop Pro103-Arg115, mutant protein displays altered kinetic profiles, primarily a lack of response to fructose-1,6-bisphosphate | Escherichia coli |
W113A | mutation in loop Pro103-Arg115, mutant protein displays altered kinetic profiles, primarily a lack of response to fructose-1,6-bisphosphate | Escherichia coli |
Y114A | mutation in loop Pro103-Arg115, mutant protein displays altered kinetic profiles, primarily a lack of response to fructose-1,6-bisphosphate | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.023 | - |
alpha-D-glucose 1-phosphate | wild-type, S0.5 value, Hill coefficient 1.4, presence of 1.5 mM fructose 1,6-bisphosphate, pH 8.0, 37°C | Escherichia coli | |
0.12 | - |
alpha-D-glucose 1-phosphate | mutant Q106A, S0.5 value, Hill coefficient 1.3, presence of 1.5 mM fructose 1,6-bisphosphate, pH 8.0, 37°C | Escherichia coli | |
0.12 | - |
alpha-D-glucose 1-phosphate | mutant R107A, S0.5 value, Hill coefficient 1.0, presence of 1.5 mM fructose 1,6-bisphosphate, pH 8.0, 37°C | Escherichia coli | |
0.143 | - |
alpha-D-glucose 1-phosphate | mutant R115A, S0.5 value, Hill coefficient 1.7, presence of 1.5 mM fructose 1,6-bisphosphate, pH 8.0, 37°C | Escherichia coli | |
0.17 | - |
alpha-D-glucose 1-phosphate | mutant P103A, S0.5 value, Hill coefficient 1.4, presence of 1.5 mM fructose 1,6-bisphosphate, pH 8.0, 37°C | Escherichia coli | |
0.17 | - |
ATP | wild-type, S0.5 value, Hill coefficient 2.0, presence of 1.5 mM fructose 1,6-bisphosphate, pH 8.0, 37°C | Escherichia coli | |
0.22 | - |
alpha-D-glucose 1-phosphate | mutant P103A, S0.5 value, Hill coefficient 1.1, pH 8.0, 37°C | Escherichia coli | |
0.25 | - |
alpha-D-glucose 1-phosphate | mutant Q106A, S0.5 value, Hill coefficient 1.6, pH 8.0, 37°C | Escherichia coli | |
0.26 | - |
alpha-D-glucose 1-phosphate | mutant R107A, S0.5 value, Hill coefficient 0.9, pH 8.0, 37°C | Escherichia coli | |
0.27 | - |
alpha-D-glucose 1-phosphate | mutant Y114A, S0.5 value, Hill coefficient 0.2, presence of 1.5 mM fructose 1,6-bisphosphate, pH 8.0, 37°C | Escherichia coli | |
0.28 | - |
alpha-D-glucose 1-phosphate | mutant R115A, S0.5 value, Hill coefficient 1.7, pH 8.0, 37°C | Escherichia coli | |
0.34 | - |
alpha-D-glucose 1-phosphate | mutant W113A, S0.5 value, Hill coefficient 1.3, presence of 1.5 mM fructose 1,6-bisphosphate, pH 8.0, 37°C | Escherichia coli | |
0.37 | - |
alpha-D-glucose 1-phosphate | wild-type, S0.5 value, Hill coefficient 1.5, pH 8.0, 37°C | Escherichia coli | |
0.43 | - |
alpha-D-glucose 1-phosphate | mutant Y114A, S0.5 value, Hill coefficient 0.3, pH 8.0, 37°C | Escherichia coli | |
0.46 | - |
alpha-D-glucose 1-phosphate | mutant W113A, S0.5 value, Hill coefficient 1.7, pH 8.0, 37°C | Escherichia coli | |
0.81 | - |
ATP | mutant P103A, S0.5 value, Hill coefficient 1.5, presence of 1.5 mM fructose 1,6-bisphosphate, pH 8.0, 37°C | Escherichia coli | |
0.89 | - |
ATP | mutant P103A, S0.5 value, Hill coefficient 1.9, pH 8.0, 37°C | Escherichia coli | |
1.3 | - |
ATP | mutant R115A, S0.5 value, Hill coefficient 1.7, presence of 1.5 mM fructose 1,6-bisphosphate, pH 8.0, 37°C | Escherichia coli | |
1.63 | - |
ATP | mutant R107A, S0.5 value, Hill coefficient 2.3, presence of 1.5 mM fructose 1,6-bisphosphate, pH 8.0, 37°C | Escherichia coli | |
2.3 | - |
ATP | mutant Y114A, S0.5 value, Hill coefficient 1.9, presence of 1.5 mM fructose 1,6-bisphosphate, pH 8.0, 37°C | Escherichia coli | |
2.5 | - |
ATP | mutant Q106A, S0.5 value, Hill coefficient 1.7, pH 8.0, 37°C | Escherichia coli | |
2.5 | - |
ATP | mutant R107A, S0.5 value, Hill coefficient 2.1, pH 8.0, 37°C | Escherichia coli | |
2.5 | - |
ATP | mutant Y114A, S0.5 value, Hill coefficient 2.0, pH 8.0, 37°C | Escherichia coli | |
2.5 | - |
ATP | wild-type, S0.5 value, Hill coefficient 2.4, pH 8.0, 37°C | Escherichia coli | |
3 | - |
ATP | mutant R115A, S0.5 value, Hill coefficient 2.1, pH 8.0, 37°C | Escherichia coli | |
3.1 | - |
ATP | mutant W113A, S0.5 value, Hill coefficient 1.1, presence of 1.5 mM fructose 1,6-bisphosphate, pH 8.0, 37°C | Escherichia coli | |
3.5 | - |
ATP | mutant W113A, S0.5 value, Hill coefficient 1.2, pH 8.0, 37°C | Escherichia coli | |
4.4 | - |
ATP | mutant Q106A, S0.5 value, Hill coefficient 1.2, presence of 1.5 mM fructose 1,6-bisphosphate, pH 8.0, 37°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + alpha-D-glucose 1-phosphate | - |
Escherichia coli | diphosphate + ADP-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | a Pro103-Arg115 loop is part of an activation path. It has strongly correlated movements with regions of the enzyme associated with regulation and ATP binding, and the optimal network pathways linking ATP and the activator binding Lys39 mainly involve residues of this loop. The loop is a distinct insertional element present only in allosterically regulated sugar nucleotide diphosphorylases | Escherichia coli |