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Literature summary for 2.7.7.27 extracted from

  • Wakuta, S.; Shibata, Y.; Yoshizaki, Y.; Saburi, W.; Hamada, S.; Ito, H.; Hwang, S.K.; Okita, T.W.; Matsui, H.
    Modulation of allosteric regulation by E38K and G101N mutations in the potato tuber ADP-glucose pyrophosphorylase (2013), Biosci. Biotechnol. Biochem., 77, 1854-1859.
    View publication on PubMed
Search for more literature for 2.7.7.27

Activating Compound

Activating Compound Comment Organism Structure
3-phosphoglycerate activation. A0.5 value and Hill coefficient for wild-type 0.076 mM and 1.0, large subunit mutant E38K 0.013 mM and 0.9, large subunit mutant G101N 0.023 mM and 1.0, large subunit mutant E38K/G101N 0.006 mM and 0.4 Solanum tuberosum

Protein Variants

Protein Variants Comment Organism
E38K the enzyme activities of large subunit mutants E38K, G101N, and E38K/G101N are more readily stimulated by phosphate-ester metabolites, such as fructose 6-phosphate, fructose 2,6-bisphosphate, and ribose 5-phosphate, than that of wild-type Solanum tuberosum
E38K/G101N in an Escherichia coli mutant defective in the synthesis of ADP-glucose, expression of large subunit mutant E38K/G101N mediates higher glycogen production than wild-type potato AGPase and the single mutant enzymes, E38K and G101N, individually. Purified large subunit mutant E38K/G101N shows higher sensitivity to 3-phosphoglycerate activation and tolerance to phosphate inhibition than mutants E38K or G101N. The enzyme activities of mutants E38K, G101N, and E38K/G101N are more readily stimulated by phosphate-ester metabolites, such as fructose 6-phosphate, fructose 2,6-bisphosphate, and ribose 5-phosphate, than that of wild-type Solanum tuberosum
G101N the enzyme activities of large subunit mutants E38K, G101N, and E38K/G101N are more readily stimulated by phosphate-ester metabolites, such as fructose 6-phosphate, fructose 2,6-bisphosphate, and ribose 5-phosphate, than that of wild-type Solanum tuberosum

Inhibitors

Inhibitors Comment Organism Structure
phosphate
-
 Solanum tuberosum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.074
-
 alpha-D-glucose 1-phosphate large subunit mutant G101N, pH 7.5, 37°C Solanum tuberosum
0.081
-
 alpha-D-glucose 1-phosphate large subunit mutant E38K/G101N, pH 7.5, 37°C Solanum tuberosum
0.11
-
 ATP large subunit mutant E38K/G101N, pH 7.5, 37°C Solanum tuberosum
0.14
-
 ATP wild-type, pH 7.5, 37°C Solanum tuberosum
0.15
-
 alpha-D-glucose 1-phosphate wild-type, pH 7.5, 37°C Solanum tuberosum
0.16
-
 ATP large subunit mutant E38K, pH 7.5, 37°C Solanum tuberosum
0.17
-
 alpha-D-glucose 1-phosphate large subunit mutant E38K, pH 7.5, 37°C Solanum tuberosum
0.17
-
 ATP large subunit mutant G101N, pH 7.5, 37°C Solanum tuberosum

Organism

Organism UniProt Comment Textmining
Solanum tuberosum Q00081 large subunit
-

Source Tissue

Source Tissue Comment Organism Textmining
tuber
-
 Solanum tuberosum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + alpha-D-glucose 1-phosphate
-
 Solanum tuberosum diphosphate + ADP-glucose
-
 ?

Synonyms

Synonyms Comment Organism
AGPS1
-
 Solanum tuberosum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
101
-
 alpha-D-glucose 1-phosphate large subunit mutant G101N, pH 7.5, 37°C Solanum tuberosum
105
-
 alpha-D-glucose 1-phosphate large subunit mutant E38K/G101N, pH 7.5, 37°C Solanum tuberosum
117
-
 ATP large subunit mutant E38K/G101N, pH 7.5, 37°C Solanum tuberosum
140
-
 ATP large subunit mutant E38K, pH 7.5, 37°C Solanum tuberosum
142
-
 alpha-D-glucose 1-phosphate large subunit mutant E38K, pH 7.5, 37°C Solanum tuberosum
148
-
 ATP large subunit mutant G101N, pH 7.5, 37°C Solanum tuberosum
204
-
 ATP wild-type, pH 7.5, 37°C Solanum tuberosum
266
-
 alpha-D-glucose 1-phosphate wild-type, pH 7.5, 37°C Solanum tuberosum

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.9
-
 wild-type, pH 7.5, 37°C Solanum tuberosum phosphate
2.6
-
 large subunit mutant G101N, pH 7.5, 37°C Solanum tuberosum phosphate
3.7
-
 large subunit mutant E38K, pH 7.5, 37°C Solanum tuberosum phosphate
8.9
-
 large subunit mutant E38K/G101N, pH 7.5, 37°C Solanum tuberosum phosphate

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
819
-
 alpha-D-glucose 1-phosphate large subunit mutant E38K, pH 7.5, 37°C Solanum tuberosum
876
-
 ATP large subunit mutant G101N, pH 7.5, 37°C Solanum tuberosum
893
-
 ATP large subunit mutant E38K, pH 7.5, 37°C Solanum tuberosum
1100
-
 ATP large subunit mutant E38K/G101N, pH 7.5, 37°C Solanum tuberosum
1300
-
 alpha-D-glucose 1-phosphate large subunit mutant E38K/G101N, pH 7.5, 37°C Solanum tuberosum
1360
-
 alpha-D-glucose 1-phosphate large subunit mutant G101N, pH 7.5, 37°C Solanum tuberosum
1460
-
 ATP wild-type, pH 7.5, 37°C Solanum tuberosum
1800
-
 alpha-D-glucose 1-phosphate wild-type, pH 7.5, 37°C Solanum tuberosum