Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Zea mays |
Protein Variants | Comment | Organism |
---|---|---|
D161G | large subunit mutant isolated by iterative saturation mutagenesis to improve heat stability of the enzyme | Zea mays |
Q96G/D161G/A443R | large subunit mutant isolated by iterative saturation mutagenesis to improve heat stability of the enzyme. Mutant additionally shows an increase affinity for activator 3-phosphoglyceric acid | Zea mays |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.038 | - |
alpha-D-glucose 1-phosphate | large subunit mutant Q96G/D161G/A443R, presence of 3-phosphoglycerate, 37°C, pH 7.4 | Zea mays | |
0.039 | - |
alpha-D-glucose 1-phosphate | wild-type, presence of 3-phosphoglycerate, 37°C, pH 7.4 | Zea mays | |
0.043 | - |
ATP | large subunit mutant D161G, 37°C, pH 7.4 | Zea mays | |
0.044 | - |
ATP | large subunit mutant D161G, presence of 3-phosphoglycerate, 37°C, pH 7.4 | Zea mays | |
0.057 | - |
ATP | large subunit mutant Q96G/D161G/A443R, presence of 3-phosphoglycerate, 37°C, pH 7.4 | Zea mays | |
0.06 | - |
alpha-D-glucose 1-phosphate | large subunit mutant D161G, presence of 3-phosphoglycerate, 37°C, pH 7.4 | Zea mays | |
0.069 | - |
ATP | wild-type, presence of 3-phosphoglycerate, 37°C, pH 7.4 | Zea mays | |
0.1 | - |
ATP | large subunit mutant Q96G/D161G/A443R, 37°C, pH 7.4 | Zea mays | |
2.8 | - |
alpha-D-glucose 1-phosphate | wild-type, 37°C, pH 7.4 | Zea mays | |
4 | - |
ATP | wild-type, 37°C, pH 7.4 | Zea mays | |
6.8 | - |
alpha-D-glucose 1-phosphate | large subunit mutant D161G, 37°C, pH 7.4 | Zea mays | |
8.74 | - |
alpha-D-glucose 1-phosphate | large subunit mutant Q96G/D161G/A443R, 37°C, pH 7.4 | Zea mays |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | Q947C0 and P55241 | Q947C0, i.e. small subunit Bt2, P55241 i.e. large subunit Sh2 | - |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.8 | - |
large subunit mutant D161G, 55°C, pH 7.4 | Zea mays |
13.1 | - |
large subunit mutant D161G, 37°C, pH 7.4 | Zea mays |
15.2 | - |
wild-type, 37°C, pH 7.4 | Zea mays |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + alpha-D-glucose 1-phosphate | - |
Zea mays | diphosphate + ADP-glucose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Brittle-2 | small subunit | Zea mays |
BT2 | small subunit | Zea mays |
SH2 | large subunit | Zea mays |
Shrunken-2 | large subunit | Zea mays |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
wild-type, complete loss of activity | Zea mays |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
35.2 | - |
ATP | wild-type, 37°C, pH 7.4 | Zea mays | |
48.8 | - |
ATP | large subunit mutant D161G, 37°C, pH 7.4 | Zea mays | |
72.4 | - |
ATP | large subunit mutant Q96G/D161G/A443R, 37°C, pH 7.4 | Zea mays |