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Literature summary for 2.7.7.27 extracted from

  • Boehlein, S.K.; Shaw, J.R.; Stewart, J.D.; Sullivan, B.; Hannah, L.C.
    Enhancing the heat stability and kinetic parameters of the maize endosperm ADP-glucose pyrophosphorylase using iterative saturation mutagenesis (2015), Arch. Biochem. Biophys., 568, 28-37.
    View publication on PubMed
Search for more literature for 2.7.7.27

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Zea mays

Protein Variants

Protein Variants Comment Organism
D161G large subunit mutant isolated by iterative saturation mutagenesis to improve heat stability of the enzyme Zea mays
Q96G/D161G/A443R large subunit mutant isolated by iterative saturation mutagenesis to improve heat stability of the enzyme. Mutant additionally shows an increase affinity for activator 3-phosphoglyceric acid Zea mays

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.038
-
 alpha-D-glucose 1-phosphate large subunit mutant Q96G/D161G/A443R, presence of 3-phosphoglycerate, 37°C, pH 7.4 Zea mays
0.039
-
 alpha-D-glucose 1-phosphate wild-type, presence of 3-phosphoglycerate, 37°C, pH 7.4 Zea mays
0.043
-
 ATP large subunit mutant D161G, 37°C, pH 7.4 Zea mays
0.044
-
 ATP large subunit mutant D161G, presence of 3-phosphoglycerate, 37°C, pH 7.4 Zea mays
0.057
-
 ATP large subunit mutant Q96G/D161G/A443R, presence of 3-phosphoglycerate, 37°C, pH 7.4 Zea mays
0.06
-
 alpha-D-glucose 1-phosphate large subunit mutant D161G, presence of 3-phosphoglycerate, 37°C, pH 7.4 Zea mays
0.069
-
 ATP wild-type, presence of 3-phosphoglycerate, 37°C, pH 7.4 Zea mays
0.1
-
 ATP large subunit mutant Q96G/D161G/A443R, 37°C, pH 7.4 Zea mays
2.8
-
 alpha-D-glucose 1-phosphate wild-type, 37°C, pH 7.4 Zea mays
4
-
 ATP wild-type, 37°C, pH 7.4 Zea mays
6.8
-
 alpha-D-glucose 1-phosphate large subunit mutant D161G, 37°C, pH 7.4 Zea mays
8.74
-
 alpha-D-glucose 1-phosphate large subunit mutant Q96G/D161G/A443R, 37°C, pH 7.4 Zea mays

Organism

Organism UniProt Comment Textmining
Zea mays Q947C0 and P55241 Q947C0, i.e. small subunit Bt2, P55241 i.e. large subunit Sh2
-

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.8
-
 large subunit mutant D161G, 55°C, pH 7.4 Zea mays
13.1
-
 large subunit mutant D161G, 37°C, pH 7.4 Zea mays
15.2
-
 wild-type, 37°C, pH 7.4 Zea mays

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + alpha-D-glucose 1-phosphate
-
 Zea mays diphosphate + ADP-glucose
-
 ?

Synonyms

Synonyms Comment Organism
Brittle-2 small subunit Zea mays
BT2 small subunit Zea mays
SH2 large subunit Zea mays
Shrunken-2 large subunit Zea mays

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 wild-type, complete loss of activity Zea mays

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
35.2
-
 ATP wild-type, 37°C, pH 7.4 Zea mays
48.8
-
 ATP large subunit mutant D161G, 37°C, pH 7.4 Zea mays
72.4
-
 ATP large subunit mutant Q96G/D161G/A443R, 37°C, pH 7.4 Zea mays