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Literature summary for 2.7.7.27 extracted from

  • Hwang, S.K.; Hamada, S.; Okita, T.W.
    ATP binding site in the plant ADP-glucose pyrophosphorylase large subunit (2006), FEBS Lett., 580, 6741-6748.
    View publication on PubMed
Search for more literature for 2.7.7.27

Activating Compound

Activating Compound Comment Organism Structure
3-phosphoglycerate 50% activation at 0.12 mM for wild-type, at 1.38 mM for mutant D157E, at 0.03 mM for mutant K41R of large subunit plus D143N of small subunit Solanum tuberosum

Protein Variants

Protein Variants Comment Organism
A132D mutation in ATP binding region of large subunit, kinetic analysis Solanum tuberosum
A132F mutation in ATP binding region of large subunit, kinetic analysis Solanum tuberosum
A132N mutation in ATP binding region of large subunit, kinetic analysis Solanum tuberosum
A132V mutation in ATP binding region of large subunit, kinetic analysis Solanum tuberosum
D157E mutation in ATP binding region of large subunit, kinetic analysis Solanum tuberosum
D157L mutation in ATP binding region of large subunit, kinetic analysis Solanum tuberosum
D157L mutation in ATP binding region of large subunit, plus mutation D143N in small subunit, kinetic analysis Solanum tuberosum
D157N mutation in ATP binding region of large subunit, kinetic analysis Solanum tuberosum
G128A mutation in ATP binding region of large subunit, kinetic analysis Solanum tuberosum
G128L mutation in ATP binding region of large subunit, kinetic analysis Solanum tuberosum
G267L mutation in ATP binding region of large subunit, kinetic analysis Solanum tuberosum
G267S mutation in ATP binding region of large subunit, kinetic analysis Solanum tuberosum
G36A mutation in ATP binding region of large subunit, kinetic analysis Solanum tuberosum
G37A mutation in ATP binding region of large subunit, kinetic analysis Solanum tuberosum
K41R mutation in ATP binding region of large subunit plus mutation D143N in small subunit, kinetic analysis Solanum tuberosum
K41R mutation in ATP binding region of large subunit, kinetic analysis Solanum tuberosum
K41R/T51K mutation in ATP binding region of large subunit, kinetic analysis Solanum tuberosum
K41R/T51K mutation in ATP binding region of large subunit, plus mutation D143N in small subunit, kinetic analysis Solanum tuberosum
Q127M mutation in ATP binding region of large subunit, kinetic analysis Solanum tuberosum
T129V mutation in ATP binding region of large subunit, kinetic analysis Solanum tuberosum
T129V/A132V mutation in ATP binding region of large subunit, kinetic analysis Solanum tuberosum
T51K mutation in ATP binding region of large subunit, kinetic analysis Solanum tuberosum
T51K mutation in ATP binding region of large subunit, plus mutation D143N in small subunit, kinetic analysis Solanum tuberosum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 alpha-D-glucose 1-phosphate wild-type Solanum tuberosum
0.12
-
 alpha-D-glucose 1-phosphate mutant D157N Solanum tuberosum
0.15
-
 alpha-D-glucose 1-phosphate mutant D157E Solanum tuberosum
0.16
-
 alpha-D-glucose 1-phosphate mutant G267S Solanum tuberosum
0.16
-
 alpha-D-glucose 1-phosphate mutant Q127M Solanum tuberosum
0.17
-
 ATP wild-type Solanum tuberosum
0.17
-
 alpha-D-glucose 1-phosphate mutation K41R/T51K, large subunit, plus D143N, small subunit Solanum tuberosum
0.18
-
 ATP mutant Q127M Solanum tuberosum
0.19
-
 ATP mutant K41R Solanum tuberosum
0.21
-
 ATP mutant G267S Solanum tuberosum
0.22
-
 alpha-D-glucose 1-phosphate mutant D157L Solanum tuberosum
0.22
-
 alpha-D-glucose 1-phosphate mutant K41R/T51K Solanum tuberosum
0.24
-
 ATP mutant G37A Solanum tuberosum
0.24
-
 alpha-D-glucose 1-phosphate mutant K41R Solanum tuberosum
0.24
-
 ATP mutant T51K Solanum tuberosum
0.25
-
 alpha-D-glucose 1-phosphate mutant G37A Solanum tuberosum
0.26
-
 ATP mutant G36A Solanum tuberosum
0.27
-
 ATP mutant K41R/T51K Solanum tuberosum
0.3
-
 ATP mutant T129V Solanum tuberosum
0.31
-
 ATP mutant D143N, small subunit Solanum tuberosum
0.32
-
 ATP mutant D157E Solanum tuberosum
0.32
-
 alpha-D-glucose 1-phosphate mutant T51K Solanum tuberosum
0.35
-
 ATP mutant A132F Solanum tuberosum
0.35
-
 ATP mutation K41R/T51K, large subunit, plus D143N, small subunit Solanum tuberosum
0.36
-
 ATP mutant A132V Solanum tuberosum
0.36
-
 ATP mutant G128A Solanum tuberosum
0.38
-
 alpha-D-glucose 1-phosphate mutation K41R, large subunit, plus D143N, small subunit Solanum tuberosum
0.39
-
 ATP mutant A132D Solanum tuberosum
0.39
-
 ATP mutant A132N Solanum tuberosum
0.4
-
 ATP mutant D157L Solanum tuberosum
0.4
-
 alpha-D-glucose 1-phosphate mutant G36A Solanum tuberosum
0.4
-
 ATP mutation K41R, large subunit, plus D143N, small subunit Solanum tuberosum
0.43
-
 ATP mutant T129V/A132V Solanum tuberosum
0.43
-
 ATP mutation T51K, large subunit, plus D143N, small subunit Solanum tuberosum
0.44
-
 alpha-D-glucose 1-phosphate mutant G128A Solanum tuberosum
0.46
-
 ATP mutant G128L Solanum tuberosum
0.47
-
 alpha-D-glucose 1-phosphate mutant G267L Solanum tuberosum
0.54
-
 alpha-D-glucose 1-phosphate mutant T129V Solanum tuberosum
0.57
-
 ATP mutant G267L Solanum tuberosum
0.58
-
 ATP mutant D157N Solanum tuberosum
0.63
-
 alpha-D-glucose 1-phosphate mutant A132N Solanum tuberosum
0.64
-
 alpha-D-glucose 1-phosphate mutant A132D Solanum tuberosum
0.71
-
 alpha-D-glucose 1-phosphate mutant G128L Solanum tuberosum
0.81
-
 alpha-D-glucose 1-phosphate mutant A132V Solanum tuberosum
0.88
-
 alpha-D-glucose 1-phosphate mutant T129V/A132V Solanum tuberosum
0.97
-
 alpha-D-glucose 1-phosphate mutant A132F Solanum tuberosum
1.01
-
 alpha-D-glucose 1-phosphate mutation T51K, large subunit, plus D143N, small subunit Solanum tuberosum

Organism

Organism UniProt Comment Textmining
Solanum tuberosum P23509
-
-

Source Tissue

Source Tissue Comment Organism Textmining
tuber
-
 Solanum tuberosum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.01
-
 mutant D143N, small subunit Solanum tuberosum
0.02
-
 mutation K41R, large subunit, plus D143N, small subunit Solanum tuberosum
1.02
-
 mutation T51K, large subunit, plus D143N, small subunit Solanum tuberosum
1.6
-
 mutant T129V/A132V Solanum tuberosum
2.9
-
 mutation K41R/T51K, large subunit, plus D143N, small subunit Solanum tuberosum
3.2
-
 mutant G128L Solanum tuberosum
5.2
-
 mutant A132F Solanum tuberosum
5.2
-
 mutant D157L Solanum tuberosum
5.4
-
 mutant A132V Solanum tuberosum
5.6
-
 mutant A132D Solanum tuberosum
6.5
-
 mutant G267L Solanum tuberosum
6.7
-
 mutant A132N Solanum tuberosum
7.6
-
 mutant G128A Solanum tuberosum
9.5
-
 mutant G37A Solanum tuberosum
13.4
-
 mutant D157E Solanum tuberosum
14.2
-
 mutant T129V Solanum tuberosum
19.1
-
 mutant D157N Solanum tuberosum
23
-
 mutant K41R/T51K Solanum tuberosum
23.7
-
 mutant T51K Solanum tuberosum
26.8
-
 mutant G267S Solanum tuberosum
37.5
-
 mutant K41R Solanum tuberosum
39.8
-
 mutant Q127M Solanum tuberosum
40.5
-
 mutant G36A Solanum tuberosum
42
-
 wild-type Solanum tuberosum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + alpha-D-glucose 1-phosphate
-
 Solanum tuberosum ADP-D-glucose + diphosphate
-
 r
diphosphate + ADP
-
 Solanum tuberosum ATP + alpha-D-glucose
-
 r

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.002
-
 alpha-D-glucose 1-phosphate mutation D157L, large subunit, plus D143N, small subunit Solanum tuberosum
0.003
-
 ATP mutation D157L, large subunit, plus D143N, small subunit Solanum tuberosum
0.03
-
 ATP mutant D143N, small subunit Solanum tuberosum
0.03
-
 alpha-D-glucose 1-phosphate mutant D143N, small subunit Solanum tuberosum
0.07
-
 ATP mutation K41R, large subunit, plus D143N, small subunit Solanum tuberosum
0.07
-
 alpha-D-glucose 1-phosphate mutation K41R, large subunit, plus D143N, small subunit Solanum tuberosum
4
-
 alpha-D-glucose 1-phosphate mutation T51K, large subunit, plus D143N, small subunit Solanum tuberosum
4.1
-
 ATP mutation T51K, large subunit, plus D143N, small subunit Solanum tuberosum
8
-
 ATP mutant T129V/A132V Solanum tuberosum
9
-
 alpha-D-glucose 1-phosphate mutation K41R/T51K, large subunit, plus D143N, small subunit Solanum tuberosum
10
-
 alpha-D-glucose 1-phosphate mutant T129V/A132V Solanum tuberosum
11
-
 alpha-D-glucose 1-phosphate mutant G128L Solanum tuberosum
11.9
-
 ATP mutation K41R/T51K, large subunit, plus D143N, small subunit Solanum tuberosum
12
-
 ATP mutant G128L Solanum tuberosum
15
-
 ATP mutant D157L Solanum tuberosum
15
-
 alpha-D-glucose 1-phosphate mutant D157L Solanum tuberosum
16
-
 alpha-D-glucose 1-phosphate mutant A132D Solanum tuberosum
16
-
 ATP mutant A132V Solanum tuberosum
18
-
 ATP mutant A132D Solanum tuberosum
19
-
 ATP mutant A132F Solanum tuberosum
21
-
 alpha-D-glucose 1-phosphate mutant A132N Solanum tuberosum
22
-
 ATP mutant A132N Solanum tuberosum
23
-
 alpha-D-glucose 1-phosphate mutant A132V Solanum tuberosum
23
-
 ATP mutant G267L Solanum tuberosum
24
-
 alpha-D-glucose 1-phosphate mutant A132F Solanum tuberosum
27
-
 alpha-D-glucose 1-phosphate mutant G267L Solanum tuberosum
33
-
 alpha-D-glucose 1-phosphate mutant D157E Solanum tuberosum
33
-
 ATP mutant G128A Solanum tuberosum
33
-
 alpha-D-glucose 1-phosphate mutant G128A Solanum tuberosum
40
-
 alpha-D-glucose 1-phosphate mutant G37A Solanum tuberosum
43
-
 ATP mutant D157E Solanum tuberosum
43
-
 ATP mutant G37A Solanum tuberosum
45
-
 ATP mutant T129V Solanum tuberosum
47
-
 alpha-D-glucose 1-phosphate mutant T129V Solanum tuberosum
63
-
 alpha-D-glucose 1-phosphate mutant D157N Solanum tuberosum
75
-
 ATP mutant D157N Solanum tuberosum
81
-
 alpha-D-glucose 1-phosphate mutant K41R/T51K Solanum tuberosum
83
-
 ATP mutant K41R/T51K Solanum tuberosum
85
-
 ATP mutant T51K Solanum tuberosum
93
-
 alpha-D-glucose 1-phosphate mutant T51K Solanum tuberosum
98
-
 alpha-D-glucose 1-phosphate mutant G267S Solanum tuberosum
107
-
 ATP mutant G267S Solanum tuberosum
133
-
 alpha-D-glucose 1-phosphate mutant G36A Solanum tuberosum
139
-
 ATP mutant K41R Solanum tuberosum
142
-
 ATP mutant G36A Solanum tuberosum
143
-
 ATP mutant Q127M Solanum tuberosum
145
-
 alpha-D-glucose 1-phosphate mutant K41R Solanum tuberosum
154
-
 alpha-D-glucose 1-phosphate mutant Q127M Solanum tuberosum
158
-
 alpha-D-glucose 1-phosphate wild-type Solanum tuberosum
166
-
 ATP wild-type Solanum tuberosum