Activating Compound | Comment | Organism | Structure |
---|---|---|---|
3-phosphoglycerate | - |
Oryza sativa | |
3-phosphoglycerate | the activities of recombinant maize and recombinant potato enzyme are comparable in the presence of 10 mM 3-phosphoglycerate, however in absence of 3-phosphoglycerate and phosphate the maize enzyme exhibits approximately 20fold more activity than does potato enzyme, in presence of both 3-phosphoglycerate and phosphate the maize enzyme is 47fold active than is the potato enzyme | Zea mays | |
3-phosphoglycerate | the activities of recombinant maize and recombinant potato enzyme are comparable in the presence of 10 mM 3-phosphoglycerate, however in absence of 3-phosphoglycerate and phosphate the maize enzyme exhibits approximately 20fold more activity than does potato enzyme, in presence of both 3-phosphoglycerate and phosphate the maize enzyme is 47fold active than is the potato enzyme | Solanum tuberosum |
Application | Comment | Organism |
---|---|---|
agriculture | genetic manipulation of enzyme to enhance rice seed yield and plant biomass | Oryza sativa |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli AC70R1-504 | Zea mays |
expression in Escherichia coli AC70R1-504 | Solanum tuberosum |
Protein Variants | Comment | Organism |
---|---|---|
L38E | UpReg-1, this mutation greatly increases activation by 3-phosphoglycerate | Zea mays |
L93Thr | Sh2-UR1, this mutation does not alter 3-phosphoglycerate activation and phosphate inhibition | Zea mays |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phosphate | - |
Oryza sativa | |
phosphate | - |
Solanum tuberosum | |
phosphate | - |
Zea mays |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
200000 | - |
- |
Zea mays |
200000 | - |
gel filtration | Solanum tuberosum |
220000 | - |
gel filtration | Zea mays |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + alpha-D-glucose 1-phosphate | Zea mays | key regulatory enzyme of starch biosynthesis | diphosphate + ADP-glucose | - |
? | |
ATP + alpha-D-glucose 1-phosphate | Solanum tuberosum | key regulatory enzyme of starch biosynthesis | diphosphate + ADP-glucose | - |
? | |
ATP + alpha-D-glucose 1-phosphate | Oryza sativa | key regulatory enzyme of starch biosynthesis | diphosphate + ADP-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | - |
- |
- |
Solanum tuberosum | - |
- |
- |
Zea mays | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
side-chain modification | substitution of two lysine residues into maize enzyme to produce a variant containing the three lysines found in the large subunit of potato enzyme has no effect on 3-phosphoglycerate activation of the maize enzyme | Zea mays |
side-chain modification | substitution of two lysine residues into maize enzyme to produce a variant containing the three lysines found in the large subunit of potato enzyme has no effect on 3-phosphoglycerate activation of the maize enzyme | Solanum tuberosum |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Zea mays |
recombinant enzyme | Solanum tuberosum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
endosperm | - |
Zea mays | - |
endosperm | - |
Solanum tuberosum | - |
endosperm | - |
Oryza sativa | - |
tuber | - |
Solanum tuberosum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + alpha-D-glucose 1-phosphate | - |
Zea mays | diphosphate + ADP-glucose | - |
? | |
ATP + alpha-D-glucose 1-phosphate | - |
Solanum tuberosum | diphosphate + ADP-glucose | - |
? | |
ATP + alpha-D-glucose 1-phosphate | - |
Oryza sativa | diphosphate + ADP-glucose | - |
? | |
ATP + alpha-D-glucose 1-phosphate | key regulatory enzyme of starch biosynthesis | Zea mays | diphosphate + ADP-glucose | - |
? | |
ATP + alpha-D-glucose 1-phosphate | key regulatory enzyme of starch biosynthesis | Solanum tuberosum | diphosphate + ADP-glucose | - |
? | |
ATP + alpha-D-glucose 1-phosphate | key regulatory enzyme of starch biosynthesis | Oryza sativa | diphosphate + ADP-glucose | - |
? |