Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.27 extracted from

  • Sikka, V.K.; Choi, S.B.; Kavakli, I.H.; Sakulsingharoj, C.; Gupta, S.; Ito, H.; Okita, T.W.
    Subcellular compartmentation and allosteric regulation of the rice endosperm ADPglucose pyrophosphorylase (2001), Plant Sci., 161, 461-468.
No PubMed abstract available
Search for more literature for 2.7.7.27

Activating Compound

Activating Compound Comment Organism Structure
3-phosphoglycerate very little enzymatic activity in the absence of 3-phosphoglycerate, 40fold in catalytic activity, when assayed und near saturating 3-phosphoglycerate concentration Oryza sativa

Inhibitors

Inhibitors Comment Organism Structure
phosphate inhibition is reversed by 3-phosphoglycerate Oryza sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.17
-
 alpha-D-glucose 1-phosphate pH 8.0, in presence of 5 mM 3-phosphoglycerate Oryza sativa
0.18
-
 ATP pH 8.0, in presence of 5 mM 3-phosphoglycerate Oryza sativa

Localization

Localization Comment Organism GeneOntology No. Textmining
amyloplast 10% of the enzyme activity Oryza sativa 9501
-
cytosol 90% of enzyme activity Oryza sativa 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requirement Oryza sativa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + alpha-D-glucose 1-phosphate Oryza sativa first step of starch biosynthesis diphosphate + ADP-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial, heat schock step at 60°C for 5 min Oryza sativa

Source Tissue

Source Tissue Comment Organism Textmining
endosperm
-
 Oryza sativa
-
leaf
-
 Oryza sativa
-
seed developing seeds Oryza sativa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + alpha-D-glucose 1-phosphate
-
 Oryza sativa diphosphate + ADP-glucose
-
 ?
ATP + alpha-D-glucose 1-phosphate first step of starch biosynthesis Oryza sativa diphosphate + ADP-glucose
-
 ?

Subunits

Subunits Comment Organism
More a major 54000 Da polypeptide and a minor 48000 Da polypeptide in the cytoplasmic fraction, a 54000 Da polypeptide in the amyloplast fraction Oryza sativa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 heat stable at 60°C Oryza sativa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.19
-
 phosphate pH 8.0, in presence of 0.5 mM 3-phosphoglycerate Oryza sativa
0.4
-
 phosphate pH 8.0, in presence of 1 mM 3-phosphoglycerate Oryza sativa