Any feedback?
Literature summary for 2.7.7.27 extracted from
- Isolation and characterization of a higher plant ADP-glucose pyrophosphorylase small subunit homotetramer (2000), FEBS Lett., 482, 113-118.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 3-phosphoglycerate | - |
Zea mays |  |
| 3-phosphoglycerate | - |
Solanum tuberosum | |
| D-fructose 1,6-bisphosphate | - |
Zea mays | |
| D-fructose 1,6-bisphosphate | - |
Solanum tuberosum | |
| D-fructose 6-phosphate | - |
Zea mays | |
| D-fructose 6-phosphate | - |
Solanum tuberosum | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| wild-type gene pML10 is mutated by hydroxylamine, the mutated SS gene TG-15 is expressed in Escherichia coli AC70R1-504 | Solanum tuberosum |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| L48F/V59I | TG-15, significant alteration in effector sensitivity of this homotetrameric enzyme in comparison to wild-type heterotetrameric enzyme | Solanum tuberosum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| phosphate | - |
Solanum tuberosum | |
| phosphate | - |
Zea mays | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Zea mays | |
| additional information | - |
additional information | - |
Solanum tuberosum | |
| 0.25 | - |
alpha-D-glucose 1-phosphate | pH 7.5, 37°C, mutant TG-15, kinetic study | Solanum tuberosum | |
| 1.2 | - |
ATP | pH 7.5, 37°C, mutant TG-15, kinetic study | Solanum tuberosum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Solanum tuberosum | |
| Mg2+ | requirement | Zea mays | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
amino acid composition and comparison | Zea mays |
| additional information | - |
amino acid composition and comparison | Synechocystis sp. |
| additional information | - |
amino acid composition and comparison | Solanum tuberosum |
| additional information | - |
amino acid composition and comparison | Anabaena sp. |
| additional information | - |
amino acid composition and comparison | Escherichia coli |
| 50000 | - |
4 * 50000, the small subunit enzyme TG-15, SDS-PAGE | Solanum tuberosum |
| 200000 | - |
- |
Zea mays |
| 200000 | - |
gel filtration of homotetramer small subunit enzyme TG-15 | Solanum tuberosum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Anabaena sp. | P30521 | - |
- |
| Escherichia coli | P0A6V1 | glgC | - |
| Solanum tuberosum | P23509 | SS | - |
| Solanum tuberosum SS | P23509 | SS | - |
| Synechocystis sp. | P52415 | - |
- |
| Zea mays | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial, homotetrameric small subunit enzyme TG-15, heat shock step at 55°C | Solanum tuberosum |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + alpha-D-glucose 1-phosphate = diphosphate + ADP-alpha-D-glucose | the small and large subunits of the enzyme define the catalytic and regulatory subunits | Solanum tuberosum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Solanum tuberosum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + alpha-D-glucose 1-phosphate | - |
Zea mays | diphosphate + ADP-glucose | - |
? | |
| ATP + alpha-D-glucose 1-phosphate | - |
Synechocystis sp. | diphosphate + ADP-glucose | - |
? | |
| ATP + alpha-D-glucose 1-phosphate | - |
Solanum tuberosum | diphosphate + ADP-glucose | - |
? | |
| ATP + alpha-D-glucose 1-phosphate | - |
Anabaena sp. | diphosphate + ADP-glucose | - |
? | |
| ATP + alpha-D-glucose 1-phosphate | - |
Escherichia coli | diphosphate + ADP-glucose | - |
? | |
| ATP + alpha-D-glucose 1-phosphate | - |
Solanum tuberosum SS | diphosphate + ADP-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | - |
Zea mays |
| homotetramer | 4 * 50000, the small subunit enzyme TG-15, SDS-PAGE | Solanum tuberosum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
purification of small subunit enzyme TG-15 involves a 7 min/55°C heat shock step | Solanum tuberosum |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | comparison of Ki of phosphate | Solanum tuberosum |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
