Cloned (Comment) | Organism |
---|---|
expression of the wild-type and mutant enzymes in Escherichia coli, Shrunken2, Sh2 encodes the large subunit and Brittle2, Bt2 encodes the small subunit, cloning and expression of the heat-stable variant Sh2hs33 and Bt2 in the yeast two-hybrid expression system | Zea mays |
Protein Variants | Comment | Organism |
---|---|---|
H333T | Sh2hs33, coexpression with wild-type Brittle2: 5 min 60°C heat treatment, 76% remaining activity in comparison to the 26% remaining activity of wild-type Shrunken2/Brittle2, enhanced subunit interaction in this mutant | Zea mays |
H333T/T460I | Sh2hs40, coexpression with wild-type Brittle2: 5 min 60°C heat treatment, 72% remaining activity in comparison to the 26% remaining activity of wild-type Shrunken2/Brittle2 | Zea mays |
R104T | Sh2hs16, coexpression with wild-type Brittle2: 5 min 60°C heat treatment, 37% remaining activity in comparison to the 26% remaining activity of wild-type Shrunken2/Brittle2 | Zea mays |
R217P/H333T | Sh2hs47, coexpression with wild-type Brittle2: 5min 60°C heat treatment, about 65% remaining activity in comparison to the 26% remaining activity of wild-type Shrunken2/Brittle2 | Zea mays |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Zea mays |
200000 | - |
density gradient centrifugation | Zea mays |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + alpha-D-glucose 1-phosphate | Zea mays | key regulatory enzyme of starch biosynthesis | diphosphate + ADP-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
endosperm | - |
Zea mays | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + alpha-D-glucose 1-phosphate | - |
Zea mays | diphosphate + ADP-glucose | - |
? | |
ATP + alpha-D-glucose 1-phosphate | key regulatory enzyme of starch biosynthesis | Zea mays | diphosphate + ADP-glucose | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
76% remaining activity of mutant enzyme Sh2hs33 in comparison to the 26% remaining activity of wild-type Shrunken2/Brittle2 | Zea mays |