Activating Compound | Comment | Organism | Structure |
---|---|---|---|
3-phosphoglycerate | kinetic study and comparison of activation of recombinant enzymes, the activation is more drastic for recombinant enzyme APS1/APL1 | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
six genes coding for enzyme isoforms, two small and four large subunits are cloned and expressed in Escherichia coli | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | - |
Hordeum vulgare |
additional information | coexpression of the small subunit APS1 with the different large subunits APL1, APL2, APL3 and APL4, results in heterotetramers with different regulatory and kinetic properties, APS1/APL1 shows the highest affinity for the substrates and the highest sensitivity to the allosteric effectors | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phosphate | kinetic study and comparison of activation of recombinant enzymes, the inhibition is more drastic for recombinant enzyme APS1/APL1; kinetic study and comparison of activation of recombinant enzymes, the inhibition is more drastic for recombinant enzyme APS1/APL1; kinetic study and comparison of activation of recombinant enzymes, the inhibition is more drastic for recombinant enzyme APS1/APL1; kinetic study and comparison of activation of recombinant enzymes, the inhibition is more drastic for recombinant enzyme APS1/APL1; kinetic study and comparison of activation of recombinant enzymes, the inhibition is more drastic for recombinant enzyme APS1/APL1 | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.019 | - |
alpha-D-glucose 1-phosphate | pH 7.9, APS1/APL1 in presence of 0.1 mM 3-phosphoglycerate | Arabidopsis thaliana | |
0.052 | - |
alpha-D-glucose 1-phosphate | pH 7.9, APS1/APL3 in presence of 2 mM 3-phosphoglycerate | Arabidopsis thaliana | |
0.06 | - |
alpha-D-glucose 1-phosphate | pH 7.9, APS1/APL4 in presence of 1 mM 3-phosphoglycerate | Arabidopsis thaliana | |
0.067 | - |
ATP | pH 7.9, APS1/APL1 in presence of 0.1 mM 3-phosphoglycerate | Arabidopsis thaliana | |
0.076 | - |
alpha-D-glucose 1-phosphate | pH 7.9, homotetramer APS1 in presence of 20 mM 3-phosphoglycerate | Arabidopsis thaliana | |
0.085 | - |
alpha-D-glucose 1-phosphate | pH 7.9, APS1/APL2 in presence of 4 mM 3-phosphoglycerate | Arabidopsis thaliana | |
0.094 | - |
ATP | pH 7.9, APS1/APL3 in presence of 2 mM 3-phosphoglycerate | Arabidopsis thaliana | |
0.118 | - |
ATP | - |
Arabidopsis thaliana | |
0.118 | - |
ATP | pH 7.9, APS1/APL4 in presence of 1 mM 3-phosphoglycerate | Arabidopsis thaliana | |
0.42 | - |
ATP | pH 7.9, homotetramer APS1 in presence of 20 mM 3-phosphoglycerate | Arabidopsis thaliana | |
0.57 | - |
ATP | pH 7.9, APS1/APL2 in presence of 4 mM 3-phosphoglycerate | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Hordeum vulgare |
additional information | - |
- |
Oryza sativa |
additional information | - |
- |
Solanum tuberosum |
additional information | - |
- |
Arabidopsis thaliana |
additional information | - |
- |
Solanum lycopersicum |
additional information | - |
- |
Triticum aestivum |
additional information | - |
- |
Ipomoea batatas |
additional information | - |
- |
Pisum sativum |
additional information | - |
- |
Beta vulgaris |
additional information | - |
- |
Zea mays |
additional information | - |
- |
Vicia faba |
additional information | - |
amino acid composition and comparison | Oryza sativa |
additional information | - |
amino acid composition and comparison | Synechocystis sp. |
additional information | - |
amino acid composition and comparison | Solanum tuberosum |
additional information | - |
amino acid composition and comparison | Arabidopsis thaliana |
additional information | - |
amino acid composition and comparison | Anabaena sp. |
additional information | - |
amino acid composition and comparison | Solanum lycopersicum |
additional information | - |
amino acid composition and comparison | Hordeum vulgare |
additional information | - |
amino acid composition and comparison | Triticum aestivum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Anabaena sp. | P30521 | - |
- |
Arabidopsis thaliana | P55228 | APS1 | - |
Arabidopsis thaliana | P55229 | APL1 | - |
Arabidopsis thaliana | P55230 | APL2 | - |
Arabidopsis thaliana | P55231 | APL3 | - |
Arabidopsis thaliana | Q9SIK1 | APL1 | - |
Beta vulgaris | P55232 | small subunit | - |
Beta vulgaris | P55233 | large subunit | - |
Hordeum vulgare | O04896 | barley, large subunit | - |
Hordeum vulgare | P30524 | barley, large subunit | - |
Hordeum vulgare | P55238 | barley, small subunit | - |
Ipomoea batatas | O81274 | sweet potato, small subunit | - |
Ipomoea batatas | Q42859 | sweet potato, small subunit | - |
no activity in Arabidopsis thaliana | - |
subunit APS2 | - |
Oryza sativa | O23809 | large subunit | - |
Oryza sativa | P15280 | small subunit | - |
Oryza sativa | P93430 | large subunit | - |
Oryza sativa | Q9ARH9 | small subunit | - |
Pisum sativum | Q43815 | pea, SS1 | - |
Pisum sativum | Q43816 | pea, SS2 | - |
Pisum sativum | Q43819 | pea, large subunit | - |
Solanum lycopersicum | O04924 | tomato, LS1 | - |
Solanum lycopersicum | P93229 | tomato, LS2 | - |
Solanum lycopersicum | P93230 | tomato, LS3 | - |
Solanum lycopersicum | Q42882 | tomato, small subunit | - |
Solanum tuberosum | P23509 | - |
- |
Solanum tuberosum | P55242 | - |
- |
Solanum tuberosum | P55243 | LS3 | - |
Solanum tuberosum | Q00081 | LS1 | - |
Solanum tuberosum LS1 | Q00081 | LS1 | - |
Solanum tuberosum LS3 | P55243 | LS3 | - |
Synechocystis sp. | P52415 | - |
- |
Triticum aestivum | P12299 | wheat, large subunit | - |
Triticum aestivum | P30523 | wheat, small subunit | - |
Vicia faba | P52416 | large subunit | - |
Vicia faba | P52416 | SS1 | - |
Vicia faba | P52417 | SS2 | - |
Vicia faba SS1 | P52416 | SS1 | - |
Vicia faba SS2 | P52417 | SS2 | - |
Zea mays | Q941P2 | small subunit | - |
Zea mays | Q947B9 | small subunit | - |
Zea mays | Q947C0 | small subunit | - |
Purification (Comment) | Organism |
---|---|
partial, recombinant enzymes, heat treatment step of heterotetramers formed by small subunit APS1 with any of the large subunits APL1, APL2, APL3 or APL4 at 65°C and of homotetramer APS1 at 70°C, only fractions that show activity at two concentrations of activator 3-phosphoglycerate, 0.5 and 10 mM are pooled | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | - |
Zea mays | - |
embryo | - |
Vicia faba | - |
endosperm | - |
Hordeum vulgare | - |
endosperm | - |
Oryza sativa | - |
endosperm | - |
Triticum aestivum | - |
endosperm | - |
Zea mays | - |
endosperm | - |
Vicia faba | - |
fruit | - |
Solanum lycopersicum | - |
fruit | - |
Beta vulgaris | - |
leaf | - |
Oryza sativa | - |
leaf | - |
Solanum tuberosum | - |
leaf | - |
Arabidopsis thaliana | - |
leaf | - |
Solanum lycopersicum | - |
leaf | - |
Hordeum vulgare | - |
leaf | - |
Ipomoea batatas | - |
leaf | - |
Pisum sativum | - |
leaf | - |
Beta vulgaris | - |
leaf | - |
Zea mays | - |
leaf | - |
Vicia faba | - |
leaf | sink and source leaves | Beta vulgaris | - |
leaf | sink leaves | Solanum tuberosum | - |
root | - |
Solanum lycopersicum | - |
seed | - |
Oryza sativa | - |
seed | - |
Pisum sativum | - |
seed | - |
Vicia faba | - |
stem | - |
Solanum lycopersicum | - |
stem | - |
Ipomoea batatas | - |
tuber | - |
Solanum tuberosum | - |
tuber | - |
Ipomoea batatas | - |
vascular tissue | - |
Arabidopsis thaliana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.5 | - |
recombinant enzyme APS2 plus APL3 | Arabidopsis thaliana |
3.7 | - |
recombinant enzyme APS2 plus APL1 | Arabidopsis thaliana |
5 | - |
recombinant enzyme APS1 plus APL2 | Arabidopsis thaliana |
16 | - |
recombinant enzyme, homotetramer APS1 | Arabidopsis thaliana |
17 | - |
recombinant enzyme APS1 plus APL4 | Arabidopsis thaliana |
Storage Stability | Organism |
---|---|
-80°C, stable for at least 3 months | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + alpha-D-glucose 1-phosphate | - |
Hordeum vulgare | diphosphate + ADP-glucose | - |
? | |
ATP + alpha-D-glucose 1-phosphate | - |
Oryza sativa | diphosphate + ADP-glucose | - |
? | |
ATP + alpha-D-glucose 1-phosphate | - |
Synechocystis sp. | diphosphate + ADP-glucose | - |
? | |
ATP + alpha-D-glucose 1-phosphate | - |
Solanum tuberosum | diphosphate + ADP-glucose | - |
? | |
ATP + alpha-D-glucose 1-phosphate | - |
Arabidopsis thaliana | diphosphate + ADP-glucose | - |
? | |
ATP + alpha-D-glucose 1-phosphate | - |
Anabaena sp. | diphosphate + ADP-glucose | - |
? | |
ATP + alpha-D-glucose 1-phosphate | - |
Solanum lycopersicum | diphosphate + ADP-glucose | - |
? | |
ATP + alpha-D-glucose 1-phosphate | - |
Triticum aestivum | diphosphate + ADP-glucose | - |
? | |
ATP + alpha-D-glucose 1-phosphate | - |
Ipomoea batatas | diphosphate + ADP-glucose | - |
? | |
ATP + alpha-D-glucose 1-phosphate | - |
Pisum sativum | diphosphate + ADP-glucose | - |
? | |
ATP + alpha-D-glucose 1-phosphate | - |
Beta vulgaris | diphosphate + ADP-glucose | - |
? | |
ATP + alpha-D-glucose 1-phosphate | - |
Zea mays | diphosphate + ADP-glucose | - |
? | |
ATP + alpha-D-glucose 1-phosphate | - |
Vicia faba | diphosphate + ADP-glucose | - |
? | |
ATP + alpha-D-glucose 1-phosphate | - |
Vicia faba SS1 | diphosphate + ADP-glucose | - |
? | |
ATP + alpha-D-glucose 1-phosphate | - |
Vicia faba SS2 | diphosphate + ADP-glucose | - |
? | |
ATP + alpha-D-glucose 1-phosphate | - |
Solanum tuberosum LS1 | diphosphate + ADP-glucose | - |
? | |
ATP + alpha-D-glucose 1-phosphate | - |
Solanum tuberosum LS3 | diphosphate + ADP-glucose | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
purification of heterotetramers formed by small subunit APS1 with any of the large subunits APL1, APL2, APL3, or APL4 involves a 5 min/65°C heat treatment step | Arabidopsis thaliana |
70 | - |
purification of homotetramer APS1 involves a 5 min/70°C heat treatment step | Arabidopsis thaliana |