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Literature summary for 2.7.7.27 extracted from
- Biosynthesis of bacterial glycogen IX: regulatory properties of the adenosine diphosphate glucose pyrophosphrylases of the Enterobacterieae (1971), Arch. Biochem. Biophys., 142, 675-692.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| D-fructose 1,6-bisphosphate | - |
Enterobacter cloacae |  |
| D-fructose 1,6-bisphosphate | - |
Klebsiella aerogenes | |
| D-fructose 1,6-bisphosphate | - |
Escherichia aurescens | |
| D-fructose 1,6-bisphosphate | in presence of fructose 1,6-diphosphate pH-optimum decreased, effects on Km | Salmonella enterica subsp. enterica serovar Typhimurium | |
| D-fructose 1,6-bisphosphate | in presence of fructose 1,6-diphosphate pH-optimum decreased, effects on Km | Citrobacter freundii | |
| NADPH | - |
Citrobacter freundii | |
| NADPH | activation | Salmonella enterica subsp. enterica serovar Typhimurium | |
| NADPH | activation | Enterobacter cloacae | |
| NADPH | activation | Klebsiella aerogenes | |
| NADPH | activation | Escherichia aurescens | |
| pyridoxal 5'-phosphate | activation | Salmonella enterica subsp. enterica serovar Typhimurium | |
| pyridoxal 5'-phosphate | activation | Enterobacter cloacae | |
| pyridoxal 5'-phosphate | activation | Klebsiella aerogenes | |
| pyridoxal 5'-phosphate | activation | Citrobacter freundii | |
| pyridoxal 5'-phosphate | activation | Escherichia aurescens | |
| pyridoxal 5'-phosphate | influence on Km and pH-optimum | Salmonella enterica subsp. enterica serovar Typhimurium | |
| pyridoxal 5'-phosphate | influence on Km and pH-optimum | Citrobacter freundii | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | inhibition under activated and unactivated conditions | Citrobacter freundii | |
| ADP | - |
Enterobacter cloacae | |
| ADP | - |
Escherichia aurescens | |
| ADP | - |
Klebsiella aerogenes | |
| ADP | inhibition under activated and unactivated conditions | Salmonella enterica subsp. enterica serovar Typhimurium | |
| AMP | the most potent inhibitor, inhibition under activated and unactivated conditions | Citrobacter freundii | |
| AMP | - |
Enterobacter cloacae | |
| AMP | - |
Escherichia aurescens | |
| AMP | - |
Klebsiella aerogenes | |
| AMP | the most potent inhibitor, inhibition under activated and unactivated conditions | Salmonella enterica subsp. enterica serovar Typhimurium | |
| phosphate | inhibition under activated and unactivated conditions | Citrobacter freundii | |
| phosphate | - |
Enterobacter cloacae | |
| phosphate | - |
Escherichia aurescens | |
| phosphate | - |
Klebsiella aerogenes | |
| phosphate | inhibition under activated and unactivated conditions | Salmonella enterica subsp. enterica serovar Typhimurium | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.13 | - |
alpha-D-glucose 1-phosphate | pH 8.4, 37°C, influence of activators on Km | Citrobacter freundii | |
| 0.21 | - |
alpha-D-glucose 1-phosphate | pH 8.4, 37°C, influence of activators on Km | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 0.72 | - |
ATP | pH 8.4, 37°C, influence of activators on Km | Citrobacter freundii | |
| 2.4 | - |
ATP | pH 8.4, 37°C, influence of activators on Km | Salmonella enterica subsp. enterica serovar Typhimurium | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Citrobacter freundii | - |
- |
- |
| Enterobacter cloacae | - |
- |
- |
| Escherichia aurescens | - |
- |
- |
| Klebsiella aerogenes | - |
- |
- |
| no activity in Erwinia carotovora | - |
- |
- |
| no activity in Proteus vulgaris or Erwinia carotovora | - |
- |
- |
| Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Salmonella enterica subsp. enterica serovar Typhimurium |
| partial | Citrobacter freundii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.45 | - |
- |
Citrobacter freundii |
| 0.96 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + alpha-D-glucose 1-phosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | diphosphate + ADP-glucose | - |
? | |
| ATP + alpha-D-glucose 1-phosphate | - |
Enterobacter cloacae | diphosphate + ADP-glucose | - |
? | |
| ATP + alpha-D-glucose 1-phosphate | - |
Klebsiella aerogenes | diphosphate + ADP-glucose | - |
? | |
| ATP + alpha-D-glucose 1-phosphate | - |
Citrobacter freundii | diphosphate + ADP-glucose | - |
? | |
| ATP + alpha-D-glucose 1-phosphate | - |
Escherichia aurescens | diphosphate + ADP-glucose | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pH optimums in various buffer and in presence and absence of fructose-1,6-bisphosphate | Citrobacter freundii |
| 7.5 | 8.5 | broad, Tris buffer | Salmonella enterica subsp. enterica serovar Typhimurium |
| 8 | - |
bicine buffer, HEPES buffer | Citrobacter freundii |
| 8.5 | - |
Tris buffer | Citrobacter freundii |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Ki of phosphate, ADP, AMP when NADPH, fructose 1,6-bisphosphate and pyridoxalphosphate are activators | Salmonella enterica subsp. enterica serovar Typhimurium | |
| additional information | - |
additional information | Ki of phosphate, ADP, AMP when NADPH, fructose 1,6-bisphosphate and pyridoxalphosphate are activators | Citrobacter freundii |
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