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Literature summary for 2.7.7.27 extracted from

  • Iglesias, A.A.; Kakefuda, G.; Preiss, J.
    Regulatiory and structural properties of the cyanobacterial ADPglucose pyrophophorylases (1991), Plant Physiol., 97, 1187-1195.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.27

Activating Compound

Activating Compound Comment Organism Structure
2,3-diphosphoglycerate no activation Anabaena sp.
2,3-diphosphoglycerate no activation Synechocystis sp.
2-phospho-D-glycerate activation Anabaena sp.
2-phospho-D-glycerate activation Synechocystis sp.
2-phospho-D-glycerate no activation Anabaena sp.
2-phospho-D-glycerate no activation Synechocystis sp.
3-phosphoglycerate allosteric activation Anabaena sp.
3-phosphoglycerate allosteric activation Synechocystis sp.
3-phosphoglycerate kinetics Anabaena sp.
3-phosphoglycerate kinetics Synechocystis sp.
ADP no activation Anabaena sp.
ADP no activation Synechocystis sp.
AMP no activation Anabaena sp.
AMP no activation Synechocystis sp.
D-fructose 1,6-bisphosphate slight activation Anabaena sp.
D-fructose 1,6-bisphosphate slight activation Synechocystis sp.
D-fructose 6-phosphate activation Anabaena sp.
D-fructose 6-phosphate activation Synechocystis sp.
D-fructose 6-phosphate slight activation Anabaena sp.
D-fructose 6-phosphate slight activation Synechocystis sp.
D-Glucose 1,6-bisphosphate no activation Anabaena sp.
D-Glucose 1,6-bisphosphate no activation Synechocystis sp.
dihydroxyacetone phosphate no activation Anabaena sp.
dihydroxyacetone phosphate no activation Synechocystis sp.
glyceraldehyde 3-phosphate slight activation Anabaena sp.
glyceraldehyde 3-phosphate slight activation Synechocystis sp.
additional information
-
 Anabaena sp.
additional information
-
 Synechocystis sp.
phosphoenolpyruvate activation Anabaena sp.
phosphoenolpyruvate activation Synechocystis sp.
phosphoenolpyruvate slight activation Anabaena sp.
phosphoenolpyruvate slight activation Synechocystis sp.
pyridoxal 5'-phosphate activation Anabaena sp.
pyridoxal 5'-phosphate activation Synechocystis sp.
pyruvate not Anabaena sp.
pyruvate not Synechocystis sp.

Inhibitors

Inhibitors Comment Organism Structure
ADP not Anabaena sp.
ADP not Synechocystis sp.
AMP not Anabaena sp.
AMP not Synechocystis sp.
Phenylglyoxal in the presence of 3-phosphoglycerate Anabaena sp.
Phenylglyoxal in the presence of 3-phosphoglycerate Synechocystis sp.
phosphate
-
 Anabaena sp.
phosphate
-
 Synechocystis sp.
Rabbit antiserum raised against spinach enzyme not antiserum against E. coli enzyme Anabaena sp.
Rabbit antiserum raised against spinach enzyme not antiserum against E. coli enzyme Synechocystis sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
 alpha-D-glucose 1-phosphate pH 7.0, 37°C, kinetic study Anabaena sp.
0.18
-
 alpha-D-glucose 1-phosphate pH 7.0, 37°C, kinetic study Synechocystis sp.
1.55
-
 ATP pH 7.0, 37°C, kinetic study Anabaena sp.
3.2
-
 ATP pH 7.0, 37°C, kinetic study Synechocystis sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requirement Anabaena sp.
Mg2+ requirement Synechocystis sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
 4 * 53000, SDS-PAGE Synechocystis sp.
216000
-
 gel filtration Synechocystis sp.
225000
-
 gel filtration Anabaena sp.

Organism

Organism UniProt Comment Textmining
Anabaena sp.
-
 PCC 7120, cyanobacterium
-
Synechocystis sp.
-
 PCC 6803, cyanobacterium
-

Purification (Commentary)

Purification (Comment) Organism
to near homogeneity Anabaena sp.
to near homogeneity Synechocystis sp.

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
111
-
-
 Anabaena sp.
134
-
-
 Synechocystis sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + alpha-D-glucose 1-phosphate
-
 Anabaena sp. diphosphate + ADP-glucose
-
 ?
ATP + alpha-D-glucose 1-phosphate
-
 Synechocystis sp. diphosphate + ADP-glucose
-
 ?

Subunits

Subunits Comment Organism
homotetramer
-
 Anabaena sp.
homotetramer 4 * 53000, SDS-PAGE Synechocystis sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.044
-
 phosphate pH 7.0, 37°C, in absence of 3-phosphoglycerate Anabaena sp.
0.095
-
 phosphate pH 7.0, 37°C, in absence of 3-phosphoglycerate Synechocystis sp.
0.46
-
 phosphate pH 7.0, 37°C, in presence of 2.5 mM 3-phosphoglycerate Anabaena sp.
0.57
-
 phosphate pH 7.0, 37°C, in presence of 2.5 mM 3-phosphoglycerate Synechocystis sp.