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Literature summary for 2.7.7.27 extracted from
- Purification and properties of nonproteolytic degraded ADPglucose pyrophosphorylase from maize endosperm (1987), Plant Physiol., 83, 105-112.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2,3-diphosphoglycerate | no activation | Zea mays |  |
| 2-phospho-D-glycerate | no activation | Zea mays | |
| 3-phosphoglycerate | allosteric activation | Zea mays | |
| 3-phosphoglycerate | activation | Zea mays | |
| D-fructose 1,6-bisphosphate | no activation | Zea mays | |
| D-fructose 6-phosphate | activation | Zea mays | |
| D-fructose 6-phosphate | less effective than 3-phosphoglycerate | Zea mays | |
| D-Glucose 1,6-bisphosphate | no activation | Zea mays | |
| D-glucose 6-phosphate | activation | Zea mays | |
| D-glucose 6-phosphate | less effective than 3-phosphoglycerate | Zea mays | |
| dihydroxyacetone phosphate | activation, less effective than 3-phosphoglycerate | Zea mays | |
| glyceraldehyde 3-phosphate | no activation | Zea mays | |
| additional information | not: fructose 2,6-bisphosphate, Ca2+/calmodulin | Zea mays | |
| phosphoenolpyruvate | activation | Zea mays | |
| phosphoenolpyruvate | less effective than 3-phosphoglycerate | Zea mays | |
| pyruvate | not | Zea mays | |
General Stability
| General Stability | Organism |
|---|---|
| PMSF and chymostatin prevent proteolysis during purification, not p-aminobenzamidine, N-alpha-p-tosyl-L-lysine chloromethylketone, NEM, PCMB, benzamidine, leupeptin, pepstatin or EDTA | Zea mays |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| F- | NaF | Zea mays | |
| phosphate | allosteric inhibitor | Zea mays | |
| Rabbit antiserum raised against spinach enzyme | - |
Zea mays |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetic study | Zea mays | |
| additional information | - |
additional information | comparison of Km of proteolyzed and non-proteolyzed enzyme at pH 7.4 and pH 6.8 | Zea mays |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54000 | - |
4 * 54000 SDS-PAGE | Zea mays |
| 230000 | - |
gel filtration | Zea mays |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Zea mays | - |
starchy maize | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| endosperm | Zea mays |
| partial | Zea mays |
| whole kernels | Zea mays |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| endosperm | - |
Zea mays | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 34 | - |
- |
Zea mays |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + alpha-D-glucose 1-phosphate | - |
Zea mays | diphosphate + ADP-glucose | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 54000 SDS-PAGE | Zea mays |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
- |
Zea mays |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.44 | - |
phosphate | pH 7.4, 37°C, in presence of 1 mM 3-phosphoglycerate | Zea mays | |
| 9.8 | - |
phosphate | pH 7.4, 37°C, in presence of 10 mM 3-phosphoglycerate | Zea mays | |
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