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Literature summary for 2.7.7.27 extracted from

  • Amir, J.; Cherry, J.H.
    Purification and properties of adenosine diphosphoglucose pyrophosphorylase from sweet corn (1972), Plant Physiol., 49, 893-897.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.27

Activating Compound

Activating Compound Comment Organism Structure
3-phosphoglycerate allosteric activation Zea mays
3-phosphoglycerate activation Zea mays
D-fructose 6-phosphate activation Zea mays
D-fructose 6-phosphate less effective than 3-phosphoglycerate Zea mays
D-ribose 5-phosphate activation Zea mays
D-ribose 5-phosphate less effective than 3-phosphoglycerate Zea mays

General Stability

General Stability Organism
freeze-thawing reduces activity by 70%, high ionic strength, ATP and Mg2+ stabilize Zea mays

Inhibitors

Inhibitors Comment Organism Structure
ADP
-
 Zea mays
AMP
-
 Zea mays
Cl- not at 5 mM, only at 10 mM Zea mays
diphosphate
-
 Zea mays
F- not at 5 mM, only at 10 mM Zea mays
NO3- not at 5 mM, only at 10 mM Zea mays
phosphate allosteric inhibitor Zea mays
phosphoenolpyruvate
-
 Zea mays
pyruvate
-
 Zea mays
SO42- 5 mM, weak Zea mays
UDP-glucose
-
 Zea mays

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.032
-
 ATP pH 7.4, 37°C Zea mays
0.033
-
 diphosphate pH 7.4, 37°C Zea mays
0.19
-
 alpha-D-glucose 1-phosphate pH 7.4, 37°C Zea mays
0.62
-
 ADP-glucose pH 7.4, 37°C Zea mays

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requirement Zea mays
Mg2+ 6-8 mM Zea mays
additional information no activation by Mn2+, Zn2+, NH4+, Na+ Zea mays

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + alpha-D-glucose 1-phosphate Zea mays key regulatory enzyme of starch biosynthesis diphosphate + ADP-glucose
-
 r

Organism

Organism UniProt Comment Textmining
Zea mays
-
 sweet corn, var. Golden Beauty
-

Purification (Commentary)

Purification (Comment) Organism
partial Zea mays

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
 Zea mays
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.4
-
-
 Zea mays

Storage Stability

Storage Stability Organism
-20°C, in 60% (NH4)2SO4, 15 weeks Zea mays

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + alpha-D-glucose 1-phosphate specific for ATP Zea mays diphosphate + ADP-glucose
-
 r
ATP + alpha-D-glucose 1-phosphate not: TTP Zea mays diphosphate + ADP-glucose
-
 r
ATP + alpha-D-glucose 1-phosphate no substrate is UTP Zea mays diphosphate + ADP-glucose
-
 r
ATP + alpha-D-glucose 1-phosphate key regulatory enzyme of starch biosynthesis Zea mays diphosphate + ADP-glucose
-
 r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5 ADP-glucose synthesis, Tris buffer Zea mays
7 8 ADP-glucose synthesis, HEPES buffer Zea mays

pH Range

pH Minimum pH Maximum Comment Organism
6 8.3 about half-maximal activity at pH 6.0 and 8.3, Tris buffer Zea mays
6.1 8.5 about half-maximal activity at pH 6.1 and about 80% of maximal activity at pH 8.5, HEPES buffer Zea mays

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00038
-
 diphosphate pH 7.4, 37°C Zea mays