Any feedback?
Literature summary for 2.7.7.24 extracted from
- Kinetic evaluation of glucose 1-phosphate analogues with a thymidylyltransferase using a continuous coupled enzyme assay (2015), Org. Biomol. Chem., 13, 866-875.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | modified continuous spectrophotometric assay for facile detection of diphosphate released from nucleotidylyltransfase-catalysed condensation of sugar 1-phosphates and nucleoside triphosphates to produce sugar nucleotides | Streptococcus pneumoniae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (1S,2R,3S,4S,5R,6S)-2,3,4,5,6-pentahydroxycyclohexyl dihydrogen phosphate | mixed inhibition type | Streptococcus pneumoniae |  |
| [2-[(2R,3R,4R,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl]ethyl]phosphonic acid | competitive inhibition | Streptococcus pneumoniae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.083 | - |
alpha-D-glucose 1-phosphate | cosubstrate dTTP, pH 7.5, temperature not specified in the publication | Streptococcus pneumoniae | |
| 0.16 | - |
alpha-D-glucose 1-phosphate | cosubstrate UTP, pH 7.5, temperature not specified in the publication | Streptococcus pneumoniae | |
| 0.18 | - |
[[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl]methyl]phosphonic acid | cosubstrate dTTP, pH 7.5, temperature not specified in the publication | Streptococcus pneumoniae | |
| 0.38 | - |
[[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl]methyl]phosphonic acid | cosubstrate UTP, pH 7.5, temperature not specified in the publication | Streptococcus pneumoniae | |
| 0.69 | - |
2-deoxy-2-amino-alpha-D-glucose 1-phosphate | cosubstrate dTTP, pH 7.5, temperature not specified in the publication | Streptococcus pneumoniae | |
| 0.7 | - |
2-deoxy-2-amino-alpha-D-glucose 1-phosphate | cosubstrate UTP, pH 7.5, temperature not specified in the publication | Streptococcus pneumoniae | |
| 1.1 | - |
3-deoxy-3-amino-alpha-D-glucose 1-phosphate | cosubstrate dTTP, pH 7.5, temperature not specified in the publication | Streptococcus pneumoniae | |
| 1.1 | - |
3-deoxy-3-azido-alpha-D-glucose 1-phosphate | cosubstrate dTTP, pH 7.5, temperature not specified in the publication | Streptococcus pneumoniae | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptococcus pneumoniae | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dTTP + 2-deoxy-2-amino-alpha-D-glucose 1-phosphate | - |
Streptococcus pneumoniae | diphosphate + dTDP-2-deoxy-2-amino-alpha-D-glucose | - |
? | |
| dTTP + 3-deoxy-3-amino-alpha-D-glucose 1-phosphate | - |
Streptococcus pneumoniae | diphosphate + dTDP-3-deoxy-3-amino-alpha-D-glucose | - |
? | |
| dTTP + 3-deoxy-3-azido-alpha-D-glucose 1-phosphate | - |
Streptococcus pneumoniae | diphosphate + dTDP-3-deoxy-3-azido-alpha-D-glucose | - |
? | |
| dTTP + alpha-D-glucose 1-phosphate | - |
Streptococcus pneumoniae | diphosphate + dTDP-alpha-D-glucose | - |
? | |
| dTTP + [[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl]methyl]phosphonic acid | - |
Streptococcus pneumoniae | diphosphate + dTDP-[[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl]methyl]phosphonic acid | - |
? | |
| UTP + 2-deoxy-2-amino-alpha-D-glucose 1-phosphate | - |
Streptococcus pneumoniae | diphosphate + UDP-2-deoxy-2-amino-alpha-D-glucose | - |
? | |
| UTP + alpha-D-glucose 1-phosphate | - |
Streptococcus pneumoniae | diphosphate + UDP-alpha-D-glucose | - |
? | |
| UTP + [[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl]methyl]phosphonic acid | - |
Streptococcus pneumoniae | diphosphate + UMP-[[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl]methyl]phosphonic acid | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Cps2L | - |
Streptococcus pneumoniae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0003 | - |
3-deoxy-3-azido-alpha-D-glucose 1-phosphate | cosubstrate dTTP, pH 7.5, temperature not specified in the publication | Streptococcus pneumoniae | |
| 0.01 | - |
[[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl]methyl]phosphonic acid | cosubstrate UTP, pH 7.5, temperature not specified in the publication | Streptococcus pneumoniae | |
| 0.016 | - |
alpha-D-glucose 1-phosphate | cosubstrate UTP, pH 7.5, temperature not specified in the publication | Streptococcus pneumoniae | |
| 0.08 | - |
3-deoxy-3-amino-alpha-D-glucose 1-phosphate | cosubstrate dTTP, pH 7.5, temperature not specified in the publication | Streptococcus pneumoniae | |
| 0.1 | - |
2-deoxy-2-amino-alpha-D-glucose 1-phosphate | cosubstrate UTP, pH 7.5, temperature not specified in the publication | Streptococcus pneumoniae | |
| 0.55 | - |
[[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl]methyl]phosphonic acid | cosubstrate dTTP, pH 7.5, temperature not specified in the publication | Streptococcus pneumoniae | |
| 4.5 | - |
2-deoxy-2-amino-alpha-D-glucose 1-phosphate | cosubstrate dTTP, pH 7.5, temperature not specified in the publication | Streptococcus pneumoniae | |
| 24 | - |
alpha-D-glucose 1-phosphate | cosubstrate dTTP, pH 7.5, temperature not specified in the publication | Streptococcus pneumoniae | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.2 | - |
[2-[(2R,3R,4R,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl]ethyl]phosphonic acid | pH 7.5, temperature not specified in the publication | Streptococcus pneumoniae | |
| 8.9 | - |
(1S,2R,3S,4S,5R,6S)-2,3,4,5,6-pentahydroxycyclohexyl dihydrogen phosphate | pH 7.5, temperature not specified in the publication | Streptococcus pneumoniae | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.15 | - |
2-deoxy-2-amino-alpha-D-glucose 1-phosphate | cosubstrate UTP, pH 7.5, temperature not specified in the publication | Streptococcus pneumoniae | |
| 0.3 | - |
3-deoxy-3-azido-alpha-D-glucose 1-phosphate | cosubstrate dTTP, pH 7.5, temperature not specified in the publication | Streptococcus pneumoniae | |
| 0.3 | - |
[[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl]methyl]phosphonic acid | cosubstrate UTP, pH 7.5, temperature not specified in the publication | Streptococcus pneumoniae | |
| 6.5 | - |
2-deoxy-2-amino-alpha-D-glucose 1-phosphate | cosubstrate dTTP, pH 7.5, temperature not specified in the publication | Streptococcus pneumoniae | |
| 7.2 | - |
3-deoxy-3-amino-alpha-D-glucose 1-phosphate | cosubstrate dTTP, pH 7.5, temperature not specified in the publication | Streptococcus pneumoniae | |
| 9.6 | - |
alpha-D-glucose 1-phosphate | cosubstrate UTP, pH 7.5, temperature not specified in the publication | Streptococcus pneumoniae | |
| 31 | - |
[[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl]methyl]phosphonic acid | cosubstrate dTTP, pH 7.5, temperature not specified in the publication | Streptococcus pneumoniae | |
| 280 | - |
alpha-D-glucose 1-phosphate | cosubstrate dTTP, pH 7.5, temperature not specified in the publication | Streptococcus pneumoniae | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
