Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli, protein forms inclusion bodies | Streptomyces peucetius |
Crystallization (Comment) | Organism |
---|---|
homology modeling of both isoforms DnmL and RmbA | Streptomyces peucetius |
Protein Variants | Comment | Organism |
---|---|---|
additional information | deletion of the C-terminal sequence of isoform DnmL increases the expression level of truncated DnmL, albeit without enzymic activity. Substitution of the C-terminal sequence of DnmL with that of isoform RmbA also leads to expression of the recombinant protein in soluble form. The fusion protein is catalytically active | Streptomyces peucetius |
R313S/T317V/H322A/A339S/S341H/D348H | mutation of residues to the corresponing residues of isoform RmbA, giving rise to highly similar hydrophobicity pattern with RmbA. Mutations render the protein expressed in soluble form, the mutant is catalytically active | Streptomyces peucetius |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.66 | - |
dTTP | pH 7.2, 37°C, fusion protein of isoform DnmL with C-terminal sequence of isoform RmbA | Streptomyces peucetius | |
1.72 | - |
dTTP | pH 7.2, 37°, mutant R313S/T317V/H322A/A339S/S341H/D348H | Streptomyces peucetius |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces peucetius | C6K8M0 | - |
- |
Streptomyces peucetius ATCC 27952 | C6K8M0 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTTP + alpha-D-glucose 1-phosphate | - |
Streptomyces peucetius | dTDP-alpha-D-glucose + diphosphate | - |
? | |
dTTP + alpha-D-glucose 1-phosphate | - |
Streptomyces peucetius ATCC 27952 | dTDP-alpha-D-glucose + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DnmL | - |
Streptomyces peucetius |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.1 | - |
dTTP | pH 7.2, 37°, mutant R313S/T317V/H322A/A339S/S341H/D348H | Streptomyces peucetius | |
1.15 | - |
dTTP | pH 7.2, 37°C, fusion protein of isoform DnmL with C-terminal sequence of isoform RmbA | Streptomyces peucetius |