Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli as GST-fusion protein | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
co-crystallization with dTDP-glucose or dTMP and alpha-D-glucose 1-phosphate or deoxythymidine and alpha-D-glucose 1-phosphate, hanging-drop vapour-diffusion method in 1.7 M ammonium sulfate and 0.1 M sodium acetate, pH 5.3 | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dTDP-glucose | competitive to dTTP | Escherichia coli | |
dTTP | competitive to dTDPglucose and non-competitive to alpha-D-glucose 1-phosphate | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0205 | - |
dTTP | 37°C pH 8 | Escherichia coli | |
0.034 | - |
alpha-D-glucose 1-phosphate | 37°C pH 8 | Escherichia coli | |
0.095 | - |
dTDP-glucose | 37°C pH 8 | Escherichia coli | |
0.154 | - |
diphosphate | 37°C pH 8 | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | absolute requirement, 2 mM: optimal | Escherichia coli | |
Mn2+ | - |
Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTTP + alpha-D-glucose 1-phosphate | Escherichia coli | - |
dTDP-glucose + diphosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
194 | - |
forward reaction | Escherichia coli |
360 | - |
reverse reaction | Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C at concentrations higher than 5 mg/ml stable for up to three months | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTTP + alpha-D-glucose 1-phosphate | - |
Escherichia coli | dTDP-glucose + diphosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | alpha4, crystallization studies | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.5 | broad | Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | - |
Escherichia coli |