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Literature summary for 2.7.7.23 extracted from

  • Bulik, D.A.; Lindmark, D.G.; Jarroll, E.L.
    Purification and characterization of UDP-N-acetylglucosamine pyrophosphorylase from encysting Giardia (1998), Mol. Biochem. Parasitol., 95, 135-139.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
UMP inhibits catabolic reaction Giardia intestinalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
UDP-N-acetyl-D-glucosamine 30°C Giardia intestinalis
0.57
-
diphosphate 30°C Giardia intestinalis
1.21
-
UTP 30°C Giardia intestinalis
2.25
-
N-acetyl-D-glucosamine 1-phosphate 30°C Giardia intestinalis

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ can partially replace Mg2+ Giardia intestinalis
Mg2+ required for maximum activity Giardia intestinalis
Mn2+ can partially replace Mg2+ Giardia intestinalis
Ni2+ can partially replace Mn2+ Giardia intestinalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
SDS-PAGE Giardia intestinalis
66000
-
gel filtration Giardia intestinalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphosphate + UDP-N-acetyl-D-glucosamine Giardia intestinalis amino sugar metabolism UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
r
diphosphate + UDP-N-acetyl-D-glucosamine Giardia intestinalis involved in the interconversion of various amino sugars and in the synthesis of mucopolysaccharides, glycopeptides, chitin UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
r

Organism

Organism UniProt Comment Textmining
Giardia intestinalis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Giardia intestinalis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4
-
purified enzyme, 30°C Giardia intestinalis

Storage Stability

Storage Stability Organism
-20°C, 30 mM Tris-HCl, pH 7.5, 20% glycerol, 0.1 mM dithiothreitol, 6 months, stable Giardia intestinalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + UDP-glucose
-
Giardia intestinalis UTP + D-glucose 1-phosphate
-
?
diphosphate + UDP-N-acetyl-D-galactosamine
-
Giardia intestinalis UTP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
?
diphosphate + UDP-N-acetyl-D-glucosamine
-
Giardia intestinalis UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
r
diphosphate + UDP-N-acetyl-D-glucosamine amino sugar metabolism Giardia intestinalis UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
r
diphosphate + UDP-N-acetyl-D-glucosamine involved in the interconversion of various amino sugars and in the synthesis of mucopolysaccharides, glycopeptides, chitin Giardia intestinalis UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
r

Subunits

Subunits Comment Organism
dimer 2 * 33000, SDS-PAGE, gel filtration Giardia intestinalis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37 42
-
Giardia intestinalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8 both directions Giardia intestinalis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.25
-
UMP
-
Giardia intestinalis