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Literature summary for 2.7.7.23 extracted from

  • Yamamoto, K.; Kawai, H.; Moriguchi, M.; Tochikura, T.
    Purification and charcterization of yeast UDP-N-acetylglucosamine pyrophosphorylase (1976), Agric. Biol. Chem., 40, 2275-2281.
No PubMed abstract available
Search for more literature for 2.7.7.23

Activating Compound

Activating Compound Comment Organism Structure
dithioerythritol highly stimulating Saccharomyces cerevisiae
dithiothreitol highly stimulating Saccharomyces cerevisiae

General Stability

General Stability Organism
dithiothreitol increases thermal stability Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
fluoride slight inhibition Saccharomyces cerevisiae
Mercuric chloride
-
 Saccharomyces cerevisiae
N-ethylmaleimide
-
 Saccharomyces cerevisiae
p-chloromercuribenzoate
-
 Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5
-
 diphosphate pH 7.5, 30°C Saccharomyces cerevisiae
6.1
-
 UDP-N-acetyl-D-glucosamine pH 7.5, 30°C Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ can partially replace Mg2+ Saccharomyces cerevisiae
Mg2+ inhibitory at high concentrations Saccharomyces cerevisiae
Mg2+ required for maximum activity Saccharomyces cerevisiae
Mn2+ can partially replace Mg2+ Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
 gel filtration Saccharomyces cerevisiae
40000
-
 SDS-PAGE Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphosphate + UDP-N-acetyl-D-glucosamine Saccharomyces cerevisiae amino sugar metabolism UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r
diphosphate + UDP-N-acetyl-D-glucosamine Saccharomyces cerevisiae involved in the interconversion of various amino sugars and in the synthesis of mucopolysaccharides, glycopeptides, chitin UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
15.1
-
 purified enzyme, pH 7.5, 30°C Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
-20°C, extremely unstable Saccharomyces cerevisiae
4°C, phosphate buffer, pH 7.4, 0.1 mM dithiothreitol, stable Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + UDP-N-acetyl-D-glucosamine highly specific, no activity with any other sugar nucloetide tested Saccharomyces cerevisiae UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r
diphosphate + UDP-N-acetyl-D-glucosamine amino sugar metabolism Saccharomyces cerevisiae UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r
diphosphate + UDP-N-acetyl-D-glucosamine involved in the interconversion of various amino sugars and in the synthesis of mucopolysaccharides, glycopeptides, chitin Saccharomyces cerevisiae UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.5 more active with Tris than with phosphate buffer Saccharomyces cerevisiae