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Literature summary for 2.7.7.2 extracted from

  • Torchetti, E.; Bonomi, F.; Galluccio, M.; Gianazza, E.; Giancaspero, T.; Iametti, S.; Indiveri, C.; Barile, M.
    Human FAD synthase (isoform 2): A component of the machinery that delivers FAD to apo-flavoproteins (2011), FEBS J., 278, 4435-4449.
No PubMed abstract available
Search for more literature for 2.7.7.2

Cloned(Commentary)

Cloned (Comment) Organism
His6-tagged enzyme is expressed in Escherichia coli Rosetta (DE3) cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
GDP competitive inhibition Homo sapiens
GTP uncompetitive inhibition Homo sapiens
Urea the enzymatic activity of isoform FADS2 decreases dramatically at increasing urea concentration, with a mid-point for activity loss at 2.1 m urea Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00035
-
 FMN at 37°C, in 50 mM Tris-HCl, pH 7.5 Homo sapiens
0.0153
-
 ATP at 37°C, in 50 mM Tris-HCl, pH 7.5 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Homo sapiens 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ Ca2+ gives maximum FAD synthesis equal to 62% of that measured with Mg2+ Homo sapiens
Co2+ in the presence of about 0.5 mM Co2+, the activity is almost equal to that measured with Mg2+ Homo sapiens
Mg2+ MgCl2 is strictly required for both the synthetic and diphosphorolytic activity, FAD synthesis is abolished in the absence of MgCl2 Homo sapiens
Mn2+ Mn2+ gives maximum FAD synthesis equal to 77% of that measured with Mg2+ Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56537
-
 x * 56537, isoform FADS2, calculated from amino acid sequence Homo sapiens
57000
-
 x * 57000, isoform FADS2, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q8NFF5
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-chelating column chromatography and hydroxyapatite column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + FMN
-
 Homo sapiens diphosphate + FAD
-
 r
diphosphate + FAD low reaction rate Homo sapiens ATP + FMN
-
 r

Subunits

Subunits Comment Organism
? x * 56537, isoform FADS2, calculated from amino acid sequence Homo sapiens
? x * 57000, isoform FADS2, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
FADS2 isoform Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.078
-
 FMN at 37°C, in 50 mM Tris-HCl, pH 7.5 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.8
-
 GTP at 37°C, in 50 mM Tris-HCl, pH 7.5 Homo sapiens
2.5
-
 GDP at 37°C, in 50 mM Tris-HCl, pH 7.5 Homo sapiens

pI Value

Organism Comment pI Value Maximum pI Value
Homo sapiens isoelectric focusing
-
 5.66
Homo sapiens calculated from amino acid sequence
-
 6