Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strains BL21 and Rosetta (DE3) and in Pichia pastoris strain X33 | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.068 | - |
FMN | with 24 mM Na2S2O4, in 50 mM potassium phosphate (pH 7.5), at 37°C | Homo sapiens | |
0.109 | - |
FMN | without Na2S2O4, in 50 mM potassium phosphate (pH 7.5), at 37°C | Homo sapiens | |
0.116 | - |
roseoflavin mononucleotide | with 24 mM Na2S2O4, in 50 mM potassium phosphate (pH 7.5), at 37°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-nitrilotriacetic-agarose column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + FMN | - |
Homo sapiens | diphosphate + FAD | - |
? | |
ATP + roseoflavin mononucleotide | - |
Homo sapiens | diphosphate + roseoflavin adenine dinucleotide | - |
? | |
additional information | does not use 8-demethyl-8-amino-riboflavin mononucleotide as substrate | Homo sapiens | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
FMN | without Na2S2O4, in 50 mM potassium phosphate (pH 7.5), at 37°C | Homo sapiens | |
0.07 | - |
roseoflavin mononucleotide | with 24 mM Na2S2O4, in 50 mM potassium phosphate (pH 7.5), at 37°C | Homo sapiens | |
0.08 | - |
FMN | with 24 mM Na2S2O4, in 50 mM potassium phosphate (pH 7.5), at 37°C | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
roseoflavin mononucleotide | with 24 mM Na2S2O4, in 50 mM potassium phosphate (pH 7.5), at 37°C | Homo sapiens | |
0.83 | - |
FMN | without Na2S2O4, in 50 mM potassium phosphate (pH 7.5), at 37°C | Homo sapiens | |
1.2 | - |
FMN | with 24 mM Na2S2O4, in 50 mM potassium phosphate (pH 7.5), at 37°C | Homo sapiens |