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Literature summary for 2.7.7.2 extracted from
- Flavin nucleotide metabolism in plants: monofunctional enzymes synthesize FAD in plastids (2008), J. Biol. Chem., 283, 30890-30900.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloned in Escherichia coli | Arabidopsis thaliana |
| expression in Escherichia coli | Arabidopsis thaliana |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | recombinant protein has FAD synthetase activity, but not riboflavin kinase activity | Arabidopsis thaliana |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ATP | substrate inhibition; substrate inhibition; substrate inhibition | Arabidopsis thaliana |  |
| FMN | substrate inhibition; substrate inhibition; substrate inhibition | Arabidopsis thaliana | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0111 | - |
ATP | pH 8.5 | Arabidopsis thaliana | |
| 0.0111 | - |
ATP | at pH 8.5 | Arabidopsis thaliana | |
| 0.0131 | - |
ATP | pH 8.5 | Arabidopsis thaliana | |
| 0.0131 | - |
ATP | at pH 8.5 | Arabidopsis thaliana | |
| 0.0189 | - |
FMN | pH 8.5 | Arabidopsis thaliana | |
| 0.0189 | - |
FMN | at pH 8.5 | Arabidopsis thaliana | |
| 0.0208 | - |
FMN | pH 8.5 | Arabidopsis thaliana | |
| 0.0208 | - |
FMN | at pH 8.5 | Arabidopsis thaliana | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Arabidopsis thaliana | 9507 | - |
| plastid | - |
Arabidopsis thaliana | 9536 | - |
| protoplast | - |
Arabidopsis thaliana | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28800 | - |
gel filtration | Arabidopsis thaliana |
| 28800 | - |
estimated by gel filtration | Arabidopsis thaliana |
| 29200 | - |
gel filtration | Arabidopsis thaliana |
| 29200 | - |
estimated by gel filtration | Arabidopsis thaliana |
| 33900 | - |
1 * 33900, SDS-PAGE and calculated | Arabidopsis thaliana |
| 34200 | - |
1 * 34200, SDS-PAGE and calculated | Arabidopsis thaliana |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + FMN | Arabidopsis thaliana | - |
diphosphate + FAD | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | Q0WS47 | isoform RibF1 | - |
| Arabidopsis thaliana | Q8VZR0 | - |
- |
| Arabidopsis thaliana | Q9FMW8 | - |
- |
| Arabidopsis thaliana | Q9FMW8 | This UniProt-ID has been deleted; isoform RibF1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by ion exchange chromatography and gel filtration | Arabidopsis thaliana |
| by Ni-NTA affinity chromatography, ion exchange chromatography and gel filtration | Arabidopsis thaliana |
| recombinant protein | Arabidopsis thaliana |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| bolting stage | - |
Arabidopsis thaliana | - |
| flower | - |
Arabidopsis thaliana | - |
| additional information | relative expression levels show little or no variation among the organs and at the developmental stages examined. Exceptions are mature siliques and germinated seeds, which expresse 50-100% more AtRibF1 mRNA than AtRibF2 mRNA | Arabidopsis thaliana | - |
| additional information | expression levels of AtRibF1 show little or no variation among the organs and at the developmental stages examined, 2 exceptions are mature siliques and germinated seeds, which express 50-100% more AtRibF1 mRNA, AtRibF1 is a housekeeping enzyme needed by all plant organs throughout development | Arabidopsis thaliana | - |
| additional information | expression levels of AtRibF2 show little or no variation among the organs and at the developmental stages examined, AtRibF2 are housekeeping enzyme needed by all plant organs throughout development | Arabidopsis thaliana | - |
| rosette | - |
Arabidopsis thaliana | - |
| seed | - |
Arabidopsis thaliana | - |
| seed | mature siliques and germinated seeds expresse 50-100% more AtRibF1 mRNA than AtRibF2 mRNA | Arabidopsis thaliana | - |
| seedling | - |
Arabidopsis thaliana | - |
| silique | - |
Arabidopsis thaliana | - |
| silique | mature siliques and germinated seeds expresse 50-100% more AtRibF1 mRNA than AtRibF2 mRNA | Arabidopsis thaliana | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + FMN | - |
Arabidopsis thaliana | diphosphate + FAD | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 33900, SDS-PAGE and calculated | Arabidopsis thaliana |
| monomer | 1 * 34200, SDS-PAGE and calculated | Arabidopsis thaliana |
| monomer | estimated by gel filtration | Arabidopsis thaliana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AtRibF1 | - |
Arabidopsis thaliana |
| AtRibF2 | - |
Arabidopsis thaliana |
| FAD synthetase | - |
Arabidopsis thaliana |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0107 | - |
ATP | recombinant enzyme purified under native conditions, at pH 8.5 | Arabidopsis thaliana | |
| 0.0108 | - |
FMN | recombinant enzyme purified under native conditions, at pH 8.5 | Arabidopsis thaliana | |
| 0.011 | - |
ATP | pH 8.5 | Arabidopsis thaliana | |
| 0.011 | - |
FMN | pH 8.5 | Arabidopsis thaliana | |
| 0.0128 | - |
ATP | recombinant enzyme purified under native conditions, at pH 8.5 | Arabidopsis thaliana | |
| 0.0128 | - |
FMN | recombinant enzyme purified under native conditions, at pH 8.5 | Arabidopsis thaliana | |
| 0.013 | - |
ATP | pH 8.5 | Arabidopsis thaliana | |
| 0.013 | - |
FMN | pH 8.5 | Arabidopsis thaliana | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FMN | - |
Arabidopsis thaliana | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00085 | - |
FMN | pH 8.5 | Arabidopsis thaliana | |
| 0.00085 | - |
FMN | recombinant enzyme purified under native conditions, at pH 8.5 | Arabidopsis thaliana | |
| 0.00092 | - |
FMN | pH 8.5 | Arabidopsis thaliana | |
| 0.00092 | - |
FMN | recombinant enzyme purified under native conditions, at pH 8.5 | Arabidopsis thaliana | |
| 0.013 | - |
ATP | pH 8.5 | Arabidopsis thaliana | |
| 0.01302 | - |
ATP | recombinant enzyme purified under native conditions, at pH 8.5 | Arabidopsis thaliana | |
| 0.01868 | - |
ATP | recombinant enzyme purified under native conditions, at pH 8.5 | Arabidopsis thaliana | |
| 0.0187 | - |
ATP | pH 8.5 | Arabidopsis thaliana | |
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