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Literature summary for 2.7.7.2 extracted from
- Proposed steady-state kinetic mechanism for Corynebacterium ammoniagenes FAD synthetase produced by Escherichia coli (1998), Biochemistry, 37, 9716-9723.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| vector pET-23a(+) cloned and overexpressed in Escherichia coli JM109(DE-3) | Corynebacterium ammoniagenes |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diphosphate | - |
Corynebacterium ammoniagenes |  |
| FMN | substrate inhibition | Corynebacterium ammoniagenes | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0004 | - |
FAD | pH 7.6, 25°C | Corynebacterium ammoniagenes | |
| 0.001 | - |
FMN | pH 7.6, 25°C | Corynebacterium ammoniagenes | |
| 0.037 | - |
ATP | pH 7.6, 25°C | Corynebacterium ammoniagenes | |
| 0.114 | - |
diphosphate | pH 7.6, 25°C | Corynebacterium ammoniagenes | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
- |
Corynebacterium ammoniagenes |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + FMN | Corynebacterium ammoniagenes | - |
diphosphate + FAD | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Corynebacterium ammoniagenes | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Corynebacterium ammoniagenes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + FMN | - |
Corynebacterium ammoniagenes | diphosphate + FAD | - |
r | |
| ATP + FMN | adenylation of FMN is reversible, FAD and diphosphate can be converted to FMN and ATP by the enzyme, under the conditions studied phosphorylation of riboflavin is irreversible | Corynebacterium ammoniagenes | diphosphate + FAD | - |
r | |
| ATP + riboflavin | - |
Corynebacterium ammoniagenes | ? | - |
ir | |
| additional information | bifunctional FAD synthetase which shows FMN adenylyltransferase and flavokinase activities, producing FMN ATP:riboflavin 5'-phosphotransferase EC 2.7.1.26 | Corynebacterium ammoniagenes | ? | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000075 | - |
FAD | pH 7.6, 25°C | Corynebacterium ammoniagenes | |
| 0.0000833 | - |
FMN | pH 7.6, 25°C | Corynebacterium ammoniagenes | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00064 | - |
diphosphate | pH 7.6, 25°C | Corynebacterium ammoniagenes | |
| 0.06 | - |
diphosphate | pH 7.6, 25°C | Corynebacterium ammoniagenes | |
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