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Literature summary for 2.7.7.2 extracted from

  • McCormick, D.B.; Oka, M.; Bowers-Komro, D.M.; Yamada, Y.; Hartman, H.A.
    Purification and properties of FAD synthetase from liver (1997), Methods Enzymol., 280, 407-413.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
diphosphate product inhibition Rattus norvegicus
FAD strong product inhibitor, 50% inhibition at 0.006 mM, competitive inhibition against ATP, mixed inhibition against FMN Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ about 30% of the activity with Mg2+ Rattus norvegicus
Mg2+
-
Rattus norvegicus
Mg2+
-
Bos taurus
Mn2+ about 15% of the activity with Mg2+ Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
2 * 53000, SDS-PAGE Rattus norvegicus
97000
-
gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + FMN Bos taurus
-
diphosphate + FAD
-
r
ATP + FMN Corynebacterium ammoniagenes
-
diphosphate + FAD
-
r
ATP + FMN Rattus norvegicus biosynthesis of FAD is most likely regulated by this coenzyme as a product at the stage of FAD synthetase reaction diphosphate + FAD
-
?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
bovine
-
Corynebacterium ammoniagenes
-
ATCC6872 and KY13315, previously Brevibacterium ammoniagenes
-
Rattus norvegicus
-
rat
-

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus
-
Bos taurus
-
Corynebacterium ammoniagenes

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-
liver
-
Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0062
-
-
Rattus norvegicus

Storage Stability

Storage Stability Organism
5°C, if dithiothreitol is removed from the purified enzyme solution enzyme denatures within 12 h Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + FMN
-
Rattus norvegicus diphosphate + FAD
-
r
ATP + FMN
-
Bos taurus diphosphate + FAD
-
r
ATP + FMN
-
Corynebacterium ammoniagenes diphosphate + FAD
-
r
ATP + FMN biosynthesis of FAD is most likely regulated by this coenzyme as a product at the stage of FAD synthetase reaction Rattus norvegicus diphosphate + FAD
-
?
additional information
-
Rattus norvegicus ?
-
?
additional information bifunctional FAD synthetase which shows FMN adenylyltransferase and flavokinase activities, producing FMN ATP:riboflavin 5'-phosphotransferase EC 2.7.1.26 Corynebacterium ammoniagenes ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 53000, SDS-PAGE Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
-
Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1
-
-
Bos taurus
7.1
-
-
Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
ATP nucleoside triphosphates other than ATP do not act as substrates or inhibitors Rattus norvegicus
ATP nucleoside triphosphates other than ATP do not act as substrates or inhibitors Corynebacterium ammoniagenes