Cloned (Comment) | Organism |
---|---|
ribC encodes a bifunctional flavokinase/FAD-synthetase, cloned and overexpressed in Escherichia coli BL21 | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34180 | - |
wild-type, gel filtration | Bacillus subtilis |
34200 | - |
gel filtration, nondenaturing conditions | Bacillus subtilis |
34230 | - |
mutant RibC, gel filtration | Bacillus subtilis |
35670 | - |
mass spectroscopy | Bacillus subtilis |
35670 | - |
deduced from RibC open reading frame | Bacillus subtilis |
36000 | - |
wild-type, SDS-PAGE | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + FMN | Bacillus subtilis | essential for flavin metabolism | diphosphate + FAD | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | P54575 | 1012 | - |
Bacillus subtilis 168 | P54575 | 1012 | - |
Purification (Comment) | Organism |
---|---|
wild-type and recombinant enzyme | Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.58 | - |
- |
Bacillus subtilis |
Storage Stability | Organism |
---|---|
-20°C, stable for at least 2 weeks | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + FMN | - |
Bacillus subtilis | diphosphate + FAD | - |
r | |
ATP + FMN | essential for flavin metabolism | Bacillus subtilis | diphosphate + FAD | - |
r | |
additional information | bifunctional FAD synthetase which shows FMN adenylyltransferase and flavokinase activities, producing FMN ATP:riboflavin 5'-phosphotransferase EC 2.7.1.26 | Bacillus subtilis | ? | - |
? | |
additional information | highly purified 5'-FMN is not accepted as a substrate | Bacillus subtilis | ? | - |
? | |
additional information | bifunctional FAD synthetase which shows FMN adenylyltransferase and flavokinase activities, producing FMN ATP:riboflavin 5'-phosphotransferase EC 2.7.1.26 | Bacillus subtilis 168 | ? | - |
? | |
additional information | highly purified 5'-FMN is not accepted as a substrate | Bacillus subtilis 168 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 36000, SDS-PAGE | Bacillus subtilis |