Literature summary for 2.7.7.19 extracted from

Nakel, K.; Bonneau, F.; Eckmann, C.R.; Conti, E.
Structural basis for the activation of the C. elegans noncanonical cytoplasmic poly(A)-polymerase GLD-2 by GLD-3 (2015), Proc. Natl. Acad. Sci. USA, 112, 8614-8619.

Crystallization (Commentary)

Crystallization (Comment)	Organism
germ-line development defective GLD-2GLD-3 complex up-regulates the expression of genes required for meiotic progression. The structure of a minimal polyadenylation complex that includes the conserved nucleotidyl-transferase core of GLD-2 and the N-terminal domain of GLD-3, to 2.3 A resolution, shows that the N-terminal domain of GLD-3 does not fold into the predicted multi-K homology domain but wraps around the catalytic domain of GLD-2. GLD-3 activates GLD-2 both indirectly by stabilizing the enzyme and directly by contributing positively charged residues near the RNA-binding cleft. Due to distinct structural features, GLD-2 displays unusual specificity in vitro for single-stranded RNAs with at least one adenosine at the 3'-end	Caenorhabditis elegans

Crystallization (Comment)

Organism

germ-line development defective GLD-2GLD-3 complex up-regulates the expression of genes required for meiotic progression. The structure of a minimal polyadenylation complex that includes the conserved nucleotidyl-transferase core of GLD-2 and the N-terminal domain of GLD-3, to 2.3 A resolution, shows that the N-terminal domain of GLD-3 does not fold into the predicted multi-K homology domain but wraps around the catalytic domain of GLD-2. GLD-3 activates GLD-2 both indirectly by stabilizing the enzyme and directly by contributing positively charged residues near the RNA-binding cleft. Due to distinct structural features, GLD-2 displays unusual specificity in vitro for single-stranded RNAs with at least one adenosine at the 3'-end

Caenorhabditis elegans

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Organism

Organism	UniProt	Comment	Textmining
Caenorhabditis elegans	O17087	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + RNA primer	isoform GLD-2 shows specificity in vitro for single-stranded RNAs with at least one adenosine at the 3'-end	Caenorhabditis elegans	diphosphate + RNA primer-A	-	?

Synonyms

Synonyms	Comment	Organism
GLD-2	-	Caenorhabditis elegans

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Release 2023.1 (January 2023)