Cloned (Comment) | Organism |
---|---|
genes glnmnat-a and glnmnat-b, encoded on chromosome 3 and 5, respectively, DNA and amino acid sequence determination and analysis, phylogenetic analysis of GlNMNAT isoenzymes, sequence comparisons to human isozymes, recombinant expression of His-tagged glnmnat-a gene product in Escherichia coli strain BL21 | Giardia intestinalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics are observed for NMN and ATP, but saturation is not accomplished with NAMN, implying low affinity yet detectable activity with this substrate. Double-reciprocal plots show no cooperativity for this enzyme | Giardia intestinalis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Giardia intestinalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25400 | - |
- |
Giardia intestinalis |
28100 | - |
- |
Giardia intestinalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + nicotinate beta-D-ribonucleotide | Giardia intestinalis | - |
diphosphate + deamido-NAD+ | - |
? | |
additional information | Giardia intestinalis | bifunctional enzyme, that also shows nicotinamide-nucleotide adenylyltransferase activity, EC 2.7.7.1 | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Giardia intestinalis | A8BIC5 | WB clone C6, genes glnmnat-a and glnmnat-b | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography | Giardia intestinalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + nicotinate beta-D-ribonucleotide | - |
Giardia intestinalis | diphosphate + deamido-NAD+ | - |
? | |
additional information | bifunctional enzyme, that also shows nicotinamide-nucleotide adenylyltransferase activity, EC 2.7.7.1 | Giardia intestinalis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 25400, about, isozyme GlNMNAT-A, sequence calculation, x * 28100, about, isozyme GlNMNAT-B, sequence calculation | Giardia intestinalis |
More | GlNMNAT tertiary structure model with a Rossmann-type fold, overview | Giardia intestinalis |
Synonyms | Comment | Organism |
---|---|---|
GlNMNAT | - |
Giardia intestinalis |
nicotinamide/nicotinate mononucleotide adenylyltransferase | - |
Giardia intestinalis |
NMNAT | - |
Giardia intestinalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
26 | - |
assay at | Giardia intestinalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Giardia intestinalis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Giardia intestinalis |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is essentially involved in the NAD biosynthesis via two different pathways, overview | Giardia intestinalis |