Cloned (Comment) | Organism |
---|---|
deletion construct 1-236: expressed in T.ni cell using a baculovirus expression system, expressed in Escherichia coli BL21 | Rattus norvegicus |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor-diffusion method | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
H168A | involved in catalysis | Rattus norvegicus |
H168A/Y173A | involved in catalysis | Rattus norvegicus |
additional information | deletion construct 1-236 (lacks the regulatory domain, displays constitutive activity) | Rattus norvegicus |
Y173A | involved in catalysis | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.33 | - |
choline phosphate | wild-type deletion construct 1-236, 8.8 mM CTP | Rattus norvegicus | |
0.48 | - |
choline phosphate | Y173A mutant deletion construct 1-236, 8.8 mM CTP | Rattus norvegicus | |
2.5 | - |
choline phosphate | H168A mutant deletion construct 1-236, 8.8 mM CTP | Rattus norvegicus | |
3.2 | - |
choline phosphate | H168A/Y173A mutant deletion construct 1-236, 8.8 mM CTP | Rattus norvegicus | |
7.3 | - |
CTP | wild-type deletion construct 1-236, 1.5 mM choline phosphate | Rattus norvegicus | |
7.7 | - |
CTP | H168A mutant deletion construct 1-236, 1.5 mM choline phosphate | Rattus norvegicus | |
7.7 | - |
CTP | Y173A mutant deletion construct 1-236, 1.5 mM choline phosphate | Rattus norvegicus | |
8.8 | - |
CTP | H168A/Y173A mutant deletion construct 1-236, 1.5 mM choline phosphate | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | P19836 | - |
- |
Purification (Comment) | Organism |
---|---|
CM- and Blue-Sepharose chromatography, gel filtartion | Rattus norvegicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.021 | - |
H168A/Y173A mutant deletion construct 1-236, variable CTP concentration, 1.5 mM choline phosphate | Rattus norvegicus |
0.034 | - |
H168A mutant deletion construct 1-236, variable CTP concentration, 1.5 mM choline phosphate | Rattus norvegicus |
0.055 | - |
H168A/Y173A mutant deletion construct 1-236, variable choline phosphate concentration, 8.8 mM CTP | Rattus norvegicus |
0.069 | - |
H168A mutant deletion construct 1-236, variable choline phosphate concentration, 8.8 mM CTP | Rattus norvegicus |
0.116 | - |
Y173A mutant deletion construct 1-236, variable choline phosphate concentration, 8.8 mM CTP | Rattus norvegicus |
0.167 | - |
Y173A mutant deletion construct 1-236, variable CTP concentration, 1.5 mM choline phosphate | Rattus norvegicus |
1.724 | - |
wild-type deletion construct 1-236, variable choline phosphate concentration, 8.8 mM CTP | Rattus norvegicus |
3.399 | - |
wild-type deletion construct 1-236, variable CTP concentration, 1.5 mM choline phosphate | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + choline phosphate | - |
Rattus norvegicus | diphosphate + CDP-choline | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | deletion construct 1-236, crystall structure analysis | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
CTP:phosphocholine cytidylyltransferase | - |
Rattus norvegicus |