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Literature summary for 2.7.7.15 extracted from

  • Veitch, D.P.; Gilham, D.; Cornell, R.B.
    The role of histidine residues in the HXGH site of CTP:phosphocholine cytidylyltransferase in CTP binding and catalysis (1998), Eur. J. Biochem., 255, 227-234.
    View publication on PubMed
Search for more literature for 2.7.7.15

Cloned(Commentary)

Cloned (Comment) Organism
-
 Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
H89A no enzymatic activity Rattus norvegicus
H89G no enzymatic activity Rattus norvegicus
H89N 100fold decrease in Vmax Rattus norvegicus
H89Q no enzymatic activity Rattus norvegicus
H92A no enzymatic activity Rattus norvegicus
H92G no enzymatic activity Rattus norvegicus
H92N reduced Vmax, increased Km Rattus norvegicus
H92Q mutation has only little effect on substrate binding and Vmax Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
Urea 50% inhibition of wild-type enzyme at 0.75 M, 50% inhibition of H92N mutant enzyme at 0.35 M, 50% inhibition of H92Q mutant enzyme at 0.2 M Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.34
-
 CTP wild-type, purified enzyme, 37°C Rattus norvegicus
0.37
-
 CTP H89N mutant, cytosolic enzyme, 37°C Rattus norvegicus
0.48
-
 phosphocholine wild-type, cytosolic enzyme, 37°C Rattus norvegicus
0.5
-
 CTP H92Q mutant, cytosolic enzyme, 37°C Rattus norvegicus
0.56
-
 phosphocholine wild-type, purified enzyme, 37°C Rattus norvegicus
0.63
-
 CTP wild-type, cytosolic enzyme, 37°C Rattus norvegicus
0.8
-
 CTP H92Q mutant, purified enzyme, 37°C Rattus norvegicus
0.87
-
 phosphocholine H92Q mutant, cytosolic and purified enzyme, 37°C Rattus norvegicus
0.98
-
 phosphocholine H89N mutant, cytosolic enzyme, 37°C Rattus norvegicus
1.73
-
 CTP H92N mutant, purified enzyme, 37°C Rattus norvegicus
2.4
-
 phosphocholine H92N mutant, cytosolic enzyme, 37°C Rattus norvegicus
2.65
-
 phosphocholine H92N mutant, purified enzyme, 37°C Rattus norvegicus
4.2
-
 CTP H92N mutant, cytosolic enzyme, 37°C Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Rattus norvegicus 5829
-

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Reaction

Reaction Comment Organism Reaction ID
CTP + phosphocholine = diphosphate + CDP-choline catalytic mechanism Rattus norvegicus
a

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CTP + choline phosphate
-
 Rattus norvegicus diphosphate + CDPcholine
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.5
-
 phosphocholine H92N mutant, purified enzyme, 37°C Rattus norvegicus
1.63
-
 phosphocholine H92Q mutant, purified enzyme, 37°C Rattus norvegicus
2.22
-
 phosphocholine wild-type, purified enzyme, 37°C Rattus norvegicus