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Literature summary for 2.7.7.15 extracted from

  • Campbell, H.A.; Kent, C.
    The CTP:phosphocholine cytidylyltransferase encoded by the licC gene of Streptococcus pneumoniae: cloning, expression, purification, and characterization (2001), Biochim. Biophys. Acta, 1534, 85-95.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Streptococcus pneumoniae

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 50% inhibition of recombinant enzyme at 0.32 mM, 50% inhibition of native enzyme at 0.27 mM Streptococcus pneumoniae
Mg2+ inhibitory above 20 mM Streptococcus pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.39
-
phosphocholine pH 8, 37°C Streptococcus pneumoniae
0.89
-
CTP pH 8, 37°C Streptococcus pneumoniae

Organism

Organism UniProt Comment Textmining
Streptococcus pneumoniae Q93MI4
-
-

Purification (Commentary)

Purification (Comment) Organism
homogeneity Streptococcus pneumoniae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
37
-
-
Streptococcus pneumoniae

Storage Stability

Storage Stability Organism
-20°C, stable for at least 8 months in 70% ammonium sulfate Streptococcus pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CTP + choline phosphate highly specific for CTP or dCTP Streptococcus pneumoniae diphosphate + CDPcholine
-
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Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
17.5
-
phosphocholine pH 8, 37°C Streptococcus pneumoniae