General Stability | Organism |
---|---|
freezing and thawing once causes more than 90% loss of activity | Rattus norvegicus |
purified enzyme is unstable in presence of DTT. CTP-Mg2+ or bovine serum albumin do not significantly stabilize the enzyme | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
diphosphate | L-form, pH 6.4, 37°C | Rattus norvegicus | |
0.007 | - |
diphosphate | H-form, pH 6.4, 37°C | Rattus norvegicus | |
0.167 | - |
choline phosphate | L-form, pH 6.4, 37°C | Rattus norvegicus | |
0.18 | - |
choline phosphate | H-form, pH 6.4, 37°C | Rattus norvegicus | |
0.208 | - |
CTP | L-form, pH 6.4, 37°C | Rattus norvegicus | |
0.21 | - |
CDPcholine | L-form, pH 6.4, 37°C | Rattus norvegicus | |
0.296 | - |
CTP | H-form, pH 6.4, 37°C | Rattus norvegicus | |
0.64 | - |
CDPcholine | H-form, pH 6.4, 37°C | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
200000 | - |
L-form of enzyme, gel filtration, aggregates in the cytosol to form high molecular weight species (H-form) with a median value of 1200000 | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
affinity chromatography, 2 forms: L-form and H-form, L-form: major species in fresh cytosol, H-form: consists of multiple copies of L-form | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.106 | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + choline phosphate | highly specific for phosphocholine | Rattus norvegicus | diphosphate + CDPcholine | - |
r |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Rattus norvegicus |