Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.15 extracted from

  • Weinhold, P.A.; Feldman, D.A.
    Choline-phosphate cytidylyltransferase (1992), Methods Enzymol., 209, 248-258.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Rattus norvegicus

General Stability

General Stability Organism
removal of detergents leads to rapid loss of activity and self-aggregation, high tendency to bind to glass and plastic surfaces even in the presence of detergents Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
4-chloromercuribenzoate 80% inhibition at 0.0006 mM Rattus norvegicus
5,5'-dithiobis(2-nitrobenzoate)
-
Rattus norvegicus
N-ethylmaleimide 80% inhibition at 1.5 mM; CTP and phosphocholine protect Rattus norvegicus
phosphate maximal inhibition of 60% at 120 mM Rattus norvegicus
Sphingolipids reversed by activating phospholipids Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.22
-
CTP pH 7, 37°C Rattus norvegicus
0.24
-
phosphocholine pH 7, 37°C Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Rattus norvegicus 5829
-
membrane
-
Rattus norvegicus 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44500
-
2 * 44500, SDS-PAGE Rattus norvegicus
97000
-
gel filtration, glycerol density gradient centrifugation Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
47.5
-
-
Rattus norvegicus

Storage Stability

Storage Stability Organism
-70°C, 50 mM Tris-HCl, 150 mM NaCl, 1.0 mM EDTA, 2.0 mM DTT, 0.025% NaN3, pH 7.4, 0.03% Triton X-100, 200 mM phosphate, stable for several months Rattus norvegicus
4°C, 20% loss of activity per day Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CTP + choline phosphate
-
Rattus norvegicus diphosphate + CDPcholine
-
?

Subunits

Subunits Comment Organism
dimer 2 * 44500, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Rattus norvegicus