Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Reducing agent | like DTT required | Rattus norvegicus | |
Reducing agent | cysteine or 2-mercaptoethanol are ineffective | Rattus norvegicus |
General Stability | Organism |
---|---|
bovine serum albumin, 2% w/v, 1 mM CTP and 20 mM Mg2+, or 10% v/v glycerol stabilizes | Rattus norvegicus |
enzyme in crude postmicrosomal supernatant is quite stable towards freezing and thawing, but the highly purified enzyme loses 85-90% of its activity when frozen and thawed twice | Rattus norvegicus |
omission of DTT from buffers used in the later steps of purification results in severe loss of activity | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CDP-ethanolamine | - |
Rattus norvegicus | |
CTP | at concentrations exceeding that of Mg2+ | Rattus norvegicus | |
diphosphate | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.053 | - |
CTP | pH 7.8, 37°C | Rattus norvegicus | |
0.065 | - |
Ethanolamine phosphate | pH 7.8, 37°C | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | postmicrosomal supernatant | Rattus norvegicus | 5829 | - |
additional information | - |
Rattus norvegicus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activation, 10% of the activity with Mg2+ | Rattus norvegicus | |
Co2+ | activation, 10% of the activity with Mg2+ | Rattus norvegicus | |
Mg2+ | absolute requirement for a divalent cation | Rattus norvegicus | |
Mg2+ | 5-10 mM most efficient | Rattus norvegicus | |
Mn2+ | activation, optimal concentration: 2-4 mM, 50% of the activity with Mg2+ | Rattus norvegicus | |
additional information | no activation by Ba2+, Zn2+, Cd2+, Ni2+ | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | 120000 | gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + ethanolamine phosphate | Rattus norvegicus | catalyzes a central step in phosphatidylethanolamine synthesis | diphosphate + CDP-ethanolamine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
male | - |
Rattus norvegicus | - |
Sprague-Dawley rats | - |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
CTP + ethanolamine phosphate = diphosphate + CDP-ethanolamine | ordered reaction mechanism | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.13 | - |
purified enzyme | Rattus norvegicus |
Storage Stability | Organism |
---|---|
0-4°C, pH 7.5-9.0, in presence of DTT, stable for weeks | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + ethanolamine phosphate | - |
Rattus norvegicus | diphosphate + CDP-ethanolamine | - |
r | |
CTP + ethanolamine phosphate | catalyzes a central step in phosphatidylethanolamine synthesis | Rattus norvegicus | diphosphate + CDP-ethanolamine | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 49000-50000, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
one sharp optimum at pH 7.8 and one with a lower maximal activity around pH 6.0 | Rattus norvegicus |
7.8 | - |
one sharp optimum at pH 7.8 and one with a lower maximal activity around pH 6.0 | Rattus norvegicus |