KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.035 | - |
alpha-D-mannose 1-phosphate | pH 8.2, 37°C | Arthrobacter sp. | |
0.052 | - |
GDP-mannose | pH 8.2, 37°C | Arthrobacter sp. | |
0.09 | - |
diphosphate | pH 8.2, 37°C | Arthrobacter sp. | |
0.12 | - |
dGDP-mannose | pH 8.2, 37°C | Arthrobacter sp. | |
0.13 | - |
GTP | pH 8.2, 37°C | Arthrobacter sp. | |
0.28 | - |
ITP | pH 8.2, 37°C | Arthrobacter sp. | |
1.9 | - |
Mg2+ | pH 8.2, 37°C | Arthrobacter sp. | |
3.3 | - |
IDP-mannose | pH 8.2, 37°C | Arthrobacter sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + alpha-D-mannose 1-phosphate | Arthrobacter sp. | - |
GDPmannose + diphosphate | - |
r | |
GTP + alpha-D-mannose 1-phosphate | Arthrobacter sp. NRRL B1973 | - |
GDPmannose + diphosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter sp. | - |
NRRL B1973 | - |
Arthrobacter sp. NRRL B1973 | - |
NRRL B1973 | - |
Purification (Comment) | Organism |
---|---|
protamine sulfate, Sephadex G-25, DEAE-cellulose | Arthrobacter sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.4 | - |
- |
Arthrobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dGTP + alpha-D-mannose 1-phosphate | 8% of activity with GDP-mannose | Arthrobacter sp. | dGDPmannose + diphosphate | - |
r | |
dGTP + alpha-D-mannose 1-phosphate | 8% of activity with GDP-mannose | Arthrobacter sp. NRRL B1973 | dGDPmannose + diphosphate | - |
r | |
GTP + alpha-D-mannose 1-phosphate | - |
Arthrobacter sp. | GDPmannose + diphosphate | - |
r | |
GTP + alpha-D-mannose 1-phosphate | equilibrium constant: 2.5 | Arthrobacter sp. | GDPmannose + diphosphate | - |
r | |
GTP + alpha-D-mannose 1-phosphate | - |
Arthrobacter sp. NRRL B1973 | GDPmannose + diphosphate | - |
r | |
GTP + alpha-D-mannose 1-phosphate | equilibrium constant: 2.5 | Arthrobacter sp. NRRL B1973 | GDPmannose + diphosphate | - |
r | |
GTP + alpha-D-mannose 1-phosphate | - |
Arthrobacter sp. | diphosphate + GDP-mannose | - |
? | |
GTP + alpha-D-mannose 1-phosphate | - |
Arthrobacter sp. NRRL B1973 | diphosphate + GDP-mannose | - |
? | |
ITP + alpha-D-mannose 1-phosphate | 11% of activity with GDP-mannose | Arthrobacter sp. | IDPmannose + diphosphate | - |
r | |
ITP + alpha-D-mannose 1-phosphate | 11% of activity with GDP-mannose | Arthrobacter sp. NRRL B1973 | IDPmannose + diphosphate | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Arthrobacter sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
48 | - |
30 min, 50% loss of activity | Arthrobacter sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | - |
Arthrobacter sp. |