Cloned (Comment) | Organism |
---|---|
overexpression of wild-type and mutants in strain BL21(DE3)pLysS | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
C160A | site-directed mutagenesis, 18fold reduced activity | Escherichia coli |
C160S | site-directed mutagenesis, 12fold reduced activity | Escherichia coli |
H166G | active site structure, complex of enzyme with UDP-D-galactose | Escherichia coli |
S161A | site-directed mutagenesis, 7000fold reduced activity compared to wild-type | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics at 4°C, wild-type and mutants | Escherichia coli | |
0.062 | - |
UDP-galactose | recombinant mutant S161A, pH 8.5, 27°C | Escherichia coli | |
0.072 | - |
UDP-glucose | recombinant mutant C160A, pH 8.5, 27°C | Escherichia coli | |
0.078 | - |
UDP-galactose | recombinant mutant C160A, pH 8.5, 27°C | Escherichia coli | |
0.082 | - |
UDP-glucose | recombinant mutant C160A, pH 8.5, 27°C | Escherichia coli | |
0.12 | - |
UDP-galactose | recombinant wild-type, pH 8.5, 27°C | Escherichia coli | |
0.125 | - |
alpha-D-galactose 1-phosphate | recombinant mutant C160A, pH 8.5, 27°C | Escherichia coli | |
0.14 | - |
alpha-D-glucose 1-phosphate | recombinant mutant C160A, pH 8.5, 27°C | Escherichia coli | |
0.16 | - |
alpha-D-glucose 1-phosphate | recombinant wild-type, pH 8.5, 27°C | Escherichia coli | |
0.2 | - |
alpha-D-glucose 1-phosphate | recombinant mutant S161A, pH 8.5, 27°C | Escherichia coli | |
0.2 | - |
UDP-glucose | recombinant wild-type, pH 8.5, 27°C | Escherichia coli | |
0.223 | - |
alpha-D-galactose 1-phosphate | recombinant mutant S161A, pH 8.5, 27°C | Escherichia coli | |
0.3 | - |
alpha-D-galactose 1-phosphate | recombinant wild-type, pH 8.5, 27°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | 0.7 mol per mol of subunit | Escherichia coli | |
Fe2+ | mutant C160A: 0.5 mol per mol of subunit, mutant S161A: 0.4 mol per mol of subunit | Escherichia coli | |
Zn2+ | 1.2 mol tightly bound per mol of subunit | Escherichia coli | |
Zn2+ | mutant C160A: 1.3 mol per mol of subunit, mutant S161A: 1.2 mol per mol of subunit like the wild-type | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P09148 | galT gene | - |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutants from strain BL21(DE3)pLysS to near homogeneity | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
UDP-alpha-D-glucose + alpha-D-galactose 1-phosphate = alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose | double-displacement mechanism | Escherichia coli | |
UDP-alpha-D-glucose + alpha-D-galactose 1-phosphate = alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose | active site structure, enzyme complexed with UDP-galactose | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00093 | - |
recombinant mutant S161A, cell extract | Escherichia coli |
0.023 | - |
purified recombinant mutant S161A | Escherichia coli |
9.9 | - |
recombinant mutant C160A, cell extract | Escherichia coli |
14.9 | - |
recombinant mutant C160S, cell extract | Escherichia coli |
16.3 | - |
recombinant wild-type, cell extract | Escherichia coli |
79.5 | - |
purified recombinant mutant C160A | Escherichia coli |
180 | - |
purified recombinant wild-type enzyme | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + alpha-D-galactose 1-phosphate | - |
Escherichia coli | alpha-D-glucose 1-phosphate + UDP-galactose | - |
r |
Synonyms | Comment | Organism |
---|---|---|
GALT | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
283 | - |
UDP-galactose | recombinant wild-type, reverse reaction, pH 8.5, 27°C | Escherichia coli | |
780 | - |
alpha-D-galactose 1-phosphate | recombinant wild-type, forward reaction, pH 8.5, 27°C | Escherichia coli |