Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutants in yeast | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
P185A | site-directed mutagenesis, reduced activity and reduced expression level compared to wild-type | Homo sapiens |
P185C | site-directed mutagenesis, no remaining activity, same expression level compared to wild-type | Homo sapiens |
P185D | site-directed mutagenesis, no remaining activity, same expression level compared to wild-type | Homo sapiens |
P185E | site-directed mutagenesis, reduced activity, same expression level compared to wild-type | Homo sapiens |
P185F | site-directed mutagenesis, no remaining activity, highly reduced expression level compared to wild-type | Homo sapiens |
P185G | site-directed mutagenesis, reduced activity and expression level compared to wild-type | Homo sapiens |
P185H | site-directed mutagenesis, no remaining activity, reduced expression level compared to wild-type | Homo sapiens |
P185I | site-directed mutagenesis, no remaining activity, highly reduced expression level compared to wild-type | Homo sapiens |
P185K | site-directed mutagenesis, no remaining activity, reduced expression level compared to wild-type | Homo sapiens |
P185L | site-directed mutagenesis, no remaining activity, highly reduced expression level compared to wild-type | Homo sapiens |
P185M | site-directed mutagenesis, no remaining activity, reduced expression level compared to wild-type | Homo sapiens |
P185N | site-directed mutagenesis, no remaining activity, reduced expression level compared to wild-type | Homo sapiens |
P185Q | site-directed mutagenesis, reduced activity, increased expression level compared to wild-type | Homo sapiens |
P185R | site-directed mutagenesis, no remaining activity, same expression level compared to wild-type | Homo sapiens |
P185S | site-directed mutagenesis, reduced activity, reduced expression level compared to wild-type | Homo sapiens |
P185T | site-directed mutagenesis, no remaining activity, reduced expression level compared to wild-type | Homo sapiens |
P185V | site-directed mutagenesis, no remaining activity, highly reduced expression level compared to wild-type | Homo sapiens |
P185W | site-directed mutagenesis, no remaining activity, highly reduced expression level compared to wild-type | Homo sapiens |
P185Y | site-directed mutagenesis, no remaining activity, reduced expression level compared to wild-type | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Escherichia coli | |
additional information | - |
additional information | kinetics | Homo sapiens | |
additional information | - |
additional information | wild-type and mutants in the 2-step reaction | Escherichia coli | |
additional information | - |
additional information | wild-type and mutants in the 2-step reaction | Homo sapiens | |
0.09 | - |
alpha-D-galactose 1-phosphate | recombinant mutants P185E, pH 8.7, 37°C | Homo sapiens | |
0.14 | - |
UDP-glucose | recombinant mutant P185S, pH 8.7, 37°C | Homo sapiens | |
0.15 | - |
alpha-D-galactose 1-phosphate | recombinant mutants P185Q, pH 8.7, 37°C | Homo sapiens | |
0.2 | - |
alpha-D-galactose 1-phosphate | recombinant mutants P185G, pH 8.7, 37°C | Homo sapiens | |
0.2 | - |
UDP-glucose | recombinant wild-type and mutants P185E and P185A, pH 8.7, 37°C | Homo sapiens | |
0.31 | - |
alpha-D-galactose 1-phosphate | recombinant mutants P185S, pH 8.7, 37°C | Homo sapiens | |
0.34 | - |
UDP-glucose | recombinant mutants P185G, pH 8.7, 37°C | Homo sapiens | |
0.4 | - |
UDP-glucose | recombinant mutants P185Q, pH 8.7, 37°C | Homo sapiens | |
0.42 | - |
alpha-D-galactose 1-phosphate | recombinant mutants P185A, pH 8.7, 37°C | Homo sapiens | |
0.57 | - |
alpha-D-galactose 1-phosphate | recombinant wild-type, pH 8.7, 37°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutants P185A, P185S, P185G, P185E, and P185Q to near homogeneity | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
UDP-alpha-D-glucose + alpha-D-galactose 1-phosphate = alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose | Pro185 has a critical role in facilitating the transferase reaction | Escherichia coli | |
UDP-alpha-D-glucose + alpha-D-galactose 1-phosphate = alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose | Pro185 has a critical role in facilitating the transferase reaction | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.6 | - |
purified recombinant mutant P185E | Homo sapiens |
2.6 | - |
purified recombinant mutant P185Q | Homo sapiens |
6.14 | - |
purified recombinant mutant P185S | Homo sapiens |
6.9 | - |
purified recombinant mutant P185G | Homo sapiens |
9.3 | - |
purified recombinant mutant P185A | Homo sapiens |
122 | - |
purified recombinant wild-type enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + alpha-D-galactose 1-phosphate | - |
Escherichia coli | alpha-D-glucose 1-phosphate + UDP-galactose | - |
r | |
UDP-glucose + alpha-D-galactose 1-phosphate | - |
Homo sapiens | alpha-D-glucose 1-phosphate + UDP-galactose | - |
r |
Synonyms | Comment | Organism |
---|---|---|
GALT | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
33 | - |
recombinant mutants P185G and P185A | Homo sapiens |
35 | - |
recombinant mutant P185S | Homo sapiens |
37 | - |
assay at | Homo sapiens |
40 | - |
recombinant mutants P185E, and P185Q | Homo sapiens |
45 | - |
recombinant wild-type | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.7 | - |
assay at | Homo sapiens |