Cloned (Comment) | Organism |
---|---|
overexpression in strain BL21 | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | inactivation of the enzyme by chelation of Zn2+ and Fe2+ ions, UDP-glucose protects, but alpha-D-glucose 1-phosphate does not | Escherichia coli | |
2,2'-bipyridyl | inactivation of the enzyme by chelation of Zn2+ and Fe2+ ions | Escherichia coli | |
8-hydroxyquinoline | inactivation of the enzyme by chelation of Zn2+ and Fe2+ ions | Escherichia coli | |
8-Hydroxyquinoline sulfonate | inactivation of the enzyme by chelation of Zn2+ and Fe2+ ions | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | required | Escherichia coli | |
Fe2+ | 0.7 mol per mol of subunit | Escherichia coli | |
additional information | enzyme contains no Ca, Cd, Cu, Mo, Ni, Co, Mn, As, Pb, or Se | Escherichia coli | |
Zn2+ | required | Escherichia coli | |
Zn2+ | 1.2 mol tightly bound per mol of subunit | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant from overexpressing strain BL21(DE3) | Escherichia coli |
to homogeneity | Escherichia coli |
Renatured (Comment) | Organism |
---|---|
enzyme reconstitution with different metal ions after denaturation with urea to eliminate the natively bound metal ions | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
170 | 190 | purified recombinant enzyme | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + alpha-D-galactose 1-phosphate | - |
Escherichia coli | alpha-D-glucose 1-phosphate + UDP-galactose | - |
r |