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Literature summary for 2.7.7.12 extracted from
- Purification and characterization of human erythrocyte uridylyl transferase (1981), Biochim. Biophys. Acta, 657, 374-382.
General Stability
| General Stability | Organism |
|---|---|
| freezing of dilute enzyme solution inactivates, concentration procedures lead to considerable loss of activity | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.065 | - |
UDP-glucose | pH 8.6, 37°C | Homo sapiens |  |
| 0.2 | - |
alpha-D-galactose 1-phosphate | pH 8.6, 37°C | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 46000 | - |
2 * 46000, SDS-PAGE | Homo sapiens |
| 88000 | - |
sucrose density gradient centrifugation | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
3 isozymes | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 45000fold | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| erythrocyte | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 15.1 | - |
purified enzyme | Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C, 10% loss of activity per week | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-glucose + alpha-D-galactose 1-phosphate | - |
Homo sapiens | alpha-D-glucose 1-phosphate + UDP-galactose | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 46000, SDS-PAGE | Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 44 | - |
t1/2: 2 min, 2 mg/ml serum albumin stabilizes considerably | Homo sapiens |
| 48 | - |
t1/2: 1 min, 2 mg/ml serum albumin stabilizes considerably | Homo sapiens |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Homo sapiens | 3 isozymes | - |
5 |
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