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Literature summary for 2.7.7.12 extracted from
- Galactose 1-phosphate uridyl transferase (1966), Methods Enzymol., 9, 708-713.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | - |
Bos taurus |  |
| cysteine | activation | Bos taurus | |
| glutathione | - |
Bos taurus | |
General Stability
| General Stability | Organism |
|---|---|
| SH-reagents stabilize | Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Bos taurus | |
| Cu2+ | - |
Bos taurus | |
| Mg2+ | - |
Bos taurus | |
| Mn2+ | - |
Bos taurus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no requirement for divalent cations | Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-glucose + alpha-D-galactose 1-phosphate | Homo sapiens | important step in galactose metabolism | alpha-D-glucose 1-phosphate + UDP-galactose | - |
r | |
| UDP-glucose + alpha-D-galactose 1-phosphate | Bos taurus | important step in galactose metabolism | alpha-D-glucose 1-phosphate + UDP-galactose | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
calf | - |
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| about 500fold | Homo sapiens |
| about 500fold | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| erythrocyte | - |
Homo sapiens | - |
| liver | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.058 | - |
purified enzyme | Homo sapiens |
| 12.4 | - |
purified enzyme | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CDP-glucose + alpha-D-galactose 1-phosphate | low activity | Bos taurus | alpha-D-glucose 1-phosphate + CDP-galactose | - |
? | |
| additional information | - |
Homo sapiens | ? | - |
? | |
| additional information | no activity with fructose 1-phosphate and galactose 6-phosphate | Bos taurus | ? | - |
? | |
| additional information | no activity with ADP-glucose or GDP-glucose | Bos taurus | ? | - |
? | |
| additional information | no activity with xylose 1-phosphate, and IDP-glucose | Bos taurus | ? | - |
? | |
| TDP-glucose + alpha-D-galactose 1-phosphate | 8.7% activity compared to UDP-glucose | Bos taurus | alpha-D-glucose 1-phosphate + TDP-galactose | - |
r | |
| UDP-glucose + alpha-D-galactose 1-phosphate | - |
Homo sapiens | alpha-D-glucose 1-phosphate + UDP-galactose | - |
r | |
| UDP-glucose + alpha-D-galactose 1-phosphate | - |
Bos taurus | alpha-D-glucose 1-phosphate + UDP-galactose | - |
r | |
| UDP-glucose + alpha-D-galactose 1-phosphate | important step in galactose metabolism | Homo sapiens | alpha-D-glucose 1-phosphate + UDP-galactose | - |
r | |
| UDP-glucose + alpha-D-galactose 1-phosphate | important step in galactose metabolism | Bos taurus | alpha-D-glucose 1-phosphate + UDP-galactose | - |
r | |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
t1/2: 18 h, sulfhydryl compounds stabilize | Bos taurus |
| 7 | 8 | fairly stable | Bos taurus |
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Release 2023.1 (January 2023)
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