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Literature summary for 2.7.7.10 extracted from
- Separation and characteristics of galactose-1-phosphate and glucose-1-phosphate uridyltranferase from fruit peduncles of cucumber (1981), Planta, 153, 370-375.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no effectors of UDP-galactose-forming reaction are MgUDP, magnesium citrate, magnesium oxaloacetate or sucrose | Cucumis sativus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Mg-diphosphate | strong inhibition, UDP-galactose synthesis | Cucumis sativus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | 0.14 | UTP | pH 7.2, 30°C | Cucumis sativus | |
| 0.18 | - |
diphosphate | pH 7.2, 30°C | Cucumis sativus | |
| 0.18 | - |
diphosphate | phosphate in form of Mg-diphosphate | Cucumis sativus | |
| 0.75 | - |
alpha-D-glucose 1-phosphate | pH 7.2, 30°C | Cucumis sativus | |
| 1.2 | - |
alpha-D-galactose 1-phosphate | pH 7.2, 30°C | Cucumis sativus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | requirement | Cucumis sativus | |
| Mg2+ | MgH2P2O7 | Cucumis sativus | |
| additional information | phosphate is no effector of UDP-galactose synthesis | Cucumis sativus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cucumis sativus | - |
cv. Calypiso | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Cucumis sativus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fruit peduncle | - |
Cucumis sativus | - |
| fruit peduncle | of unpollinated ovaries | Cucumis sativus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.36 | - |
peduncles of developing fruits | Cucumis sativus |
| 0.56 | - |
unpollinated peduncles | Cucumis sativus |
| 3.8 | - |
partially purified enzyme, substrate alpha-D-galactose 1-phosphate | Cucumis sativus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + UDP-alpha-D-galactose | - |
Cucumis sativus | UTP + alpha-D-galactose 1-phosphate | - |
? | |
| UTP + alpha-D-galactose 1-phosphate | preferred substrate | Cucumis sativus | diphosphate + UDP-galactose | - |
r | |
| UTP + alpha-D-glucose 1-phosphate | - |
Cucumis sativus | diphosphate + UDP-glucose | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Cucumis sativus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 56 | - |
2.5 min, 80% loss of activity in crude desalted extracts | Cucumis sativus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7.5 | broad, forward reaction | Cucumis sativus |
| 6 | - |
reverse reaction | Cucumis sativus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.58 | - |
Mg-diphosphate | pH 7.2, 30°C | Cucumis sativus | |
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