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Literature summary for 2.7.7.10 extracted from
- Galactose-1-phosphate uridylyltransferase from Entamoeba histolytica (1982), Methods Enzymol., 90, 552-555.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.034 | - |
UTP | pH 7.0, 30°C | Entamoeba histolytica |  |
| 0.23 | - |
alpha-D-glucose 1-phosphate | pH 7.0, 30°C | Entamoeba histolytica | |
| 0.415 | - |
alpha-D-galactose 1-phosphate | pH 7.0, 30°C | Entamoeba histolytica | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | activation | Entamoeba histolytica | |
| Mn2+ | activation | Entamoeba histolytica | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
gel filtration | Entamoeba histolytica |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UTP + alpha-D-galactose 1-phosphate | Entamoeba histolytica | constitutive enzyme | diphosphate + UDP-galactose | - |
r | |
| UTP + alpha-D-galactose 1-phosphate | Entamoeba histolytica K-9 / ATCC 30015 | constitutive enzyme | diphosphate + UDP-galactose | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Entamoeba histolytica | - |
- |
- |
| Entamoeba histolytica K-9 / ATCC 30015 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Entamoeba histolytica |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 135 | - |
purified enzyme | Entamoeba histolytica |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, in 20 mM imidazole-HCl buffer, pH 7, 20% v/v glycerol, 30% loss of activity within 6 weeks | Entamoeba histolytica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | no activity with ATP, ITP, CTP or GTP | Entamoeba histolytica | ? | - |
? | |
| additional information | no activity with ATP, ITP, CTP or GTP | Entamoeba histolytica K-9 / ATCC 30015 | ? | - |
? | |
| UTP + alpha-D-galactose 1-phosphate | - |
Entamoeba histolytica | diphosphate + UDP-galactose | - |
r | |
| UTP + alpha-D-galactose 1-phosphate | constitutive enzyme | Entamoeba histolytica | diphosphate + UDP-galactose | - |
r | |
| UTP + alpha-D-galactose 1-phosphate | - |
Entamoeba histolytica K-9 / ATCC 30015 | diphosphate + UDP-galactose | - |
r | |
| UTP + alpha-D-galactose 1-phosphate | constitutive enzyme | Entamoeba histolytica K-9 / ATCC 30015 | diphosphate + UDP-galactose | - |
r | |
| UTP + alpha-D-glucose 1-phosphate | reaction at 75% the rate of alpha-D-galactose 1-phosphate | Entamoeba histolytica | diphosphate + UDP-glucose | - |
r | |
| UTP + alpha-D-glucose 1-phosphate | reaction at 75% the rate of alpha-D-galactose 1-phosphate | Entamoeba histolytica K-9 / ATCC 30015 | diphosphate + UDP-glucose | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Entamoeba histolytica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Entamoeba histolytica |
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Release 2023.1 (January 2023)
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