Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) | - |
Saccharomyces cerevisiae | |
p-chloromercuribenzoate | - |
Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0008 | - |
ATP | 70°C, pH 7.4 | Saccharolobus solfataricus | |
0.0014 | - |
NMN | 70°C, pH 7.4 | Saccharolobus solfataricus | |
0.017 | - |
nicotinic acid mononucleotide | 70°C, pH 7.4 | Saccharolobus solfataricus | |
0.11 | - |
ATP | - |
Saccharomyces cerevisiae | |
0.19 | - |
NMN | - |
Saccharomyces cerevisiae | |
5 | - |
nicotinate ribonucleotide | - |
Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | chromatin-associated | Saccharomyces cerevisiae | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Saccharomyces cerevisiae | |
Co2+ | - |
Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
alpha4, 4 * 50000, SDS-PAGE, with or without 2-mercaptoethanol | Saccharomyces cerevisiae |
66000 | - |
gel filtration | Saccharolobus solfataricus |
195000 | - |
- |
Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Saccharomyces cerevisiae | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | 2% carbohydrates | Saccharomyces cerevisiae |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
- |
Saccharolobus solfataricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.35 | - |
- |
Saccharolobus solfataricus |
2.8 | - |
purification step: Matrix Gel Green A | Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
-20°C, in 20 mM HEPES buffer, pH 7.5, about 20% loss of activity within 1 month | Saccharomyces cerevisiae |
-20°C, several weeks without loss in activity | Saccharolobus solfataricus |
4°C, in 20 mM HEPES buffer, pH 7.5, about 30% loss of activity within 2 weeks | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + nicotinamide ribonucleotide | i.e. NMN or nicotinamide mononucleotide | Saccharomyces cerevisiae | diphosphate + NAD+ | - |
r | |
ATP + nicotinamide ribonucleotide | i.e. NMN or nicotinamide mononucleotide | Saccharolobus solfataricus | diphosphate + NAD+ | - |
r | |
ATP + nicotinate ribonucleotide | i.e. nicotinate mononucleotide | Saccharomyces cerevisiae | diphosphate + nicotinic acid-adenine dinucleotide | - |
r | |
ATP + nicotinate ribonucleotide | i.e. nicotinate mononucleotide | Saccharolobus solfataricus | diphosphate + nicotinic acid-adenine dinucleotide | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | alpha4, 4 * 50000, SDS-PAGE, with or without 2-mercaptoethanol | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | 8.4 | - |
Saccharomyces cerevisiae |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Saccharolobus solfataricus | FPLC chromatofocusing on a Mono P HR 5/5 column | - |
5.4 |
Saccharomyces cerevisiae | in the presence of 4 M urea, multiple pIs in its absence at pH 4.2, 4.9 and 6.2 | - |
6.2 |